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Effects of Carbon Source and Vitreoscilla Hemoglobin (VHb) on the Production of β-Galactosidase in Enterobacter aerogenes

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Abstract

At fixed concentration (0.5%), lactose and galactose acted as inducers while glucose and other tested carbon sugars showed repression effects on β-galactosidase production in Enterobacter aerogenes strain. The expression of Vitreoscilla hemoglobin gene (vgb) in this bacterial strain managed to overcome the repression effects as well as improving the induction of β-galactosidase formation by carbon sources. In parallel, the bacterial O2 consumption was increased correspondingly to the vgb induction of β-galactosidase synthesis. When Enterobacter aerogenes strains were grown at the incubation temperature 42°C, about 5-fold higher enzyme productivity was obtained than with a similar incubation at 37°C. The bacterial growth expressed as biomass yield had a different optimum temperature and was not influenced to the same extent by variations in the carbon sources. These data are discussed in terms of proposed enhancement in β-galactosidase productivity by vgb expression as well as its significance to improve the technology of whey processing.

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Acknowledgments

This work was supported in part by a Deanship of scientific research grant from Mutah University, Jordan.

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Authors and Affiliations

  1. Department of Biology, Mutah University, Karak, Mutah, P. O. Box 7, Jordan

    Khaled M. Khleifat, Muayad M. Abboud, Ahmed H. Al-Mustafa & Khalid Y. Al-Sharafa

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  1. Khaled M. Khleifat
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  2. Muayad M. Abboud
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  3. Ahmed H. Al-Mustafa
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  4. Khalid Y. Al-Sharafa
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Correspondence to Muayad M. Abboud.

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Khleifat, K.M., Abboud, M.M., Al-Mustafa, A.H. et al. Effects of Carbon Source and Vitreoscilla Hemoglobin (VHb) on the Production of β-Galactosidase in Enterobacter aerogenes . Curr Microbiol 53, 277–281 (2006). https://doi.org/10.1007/s00284-005-0466-3

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  • DOI: https://doi.org/10.1007/s00284-005-0466-3

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