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Purification and Characterization of an Alginate Lyase from Marine Bacterium Vibrio sp. Mutant Strain 510-64

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Abstract

Marine Vibrio sp. 510 was chosen as a parent strain for screening high producers of alginate lyase using the complex mutagenesis of Ethyl Methanesulphonate and UV radiation treatments. The mutant strain Vibrio sp. 510-64 was selected and its alginate lyase activity was increased by 3.87-fold (reaching 46.12 EU/mg) over that of the parent strain. An extracellular alginate lyase was purified from Vibrio sp. 510-64 cultural supernatant by successive fractionation on DEAE Sepharose FF and two steps of Superdex 75. The purified enzyme yielded a single band on SDS-PAGE with the molecular weight of 34.6 kDa. Data of the N-terminal amino acid sequence indicated that this protein might be a novel alginate lyase. The substrate specificity results demonstrated that the alginate lyase had the specificity for poly G block.

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Acknowledgments

This work was supported by a grant from “The Shandong Province Key Project of China” (Project No. 003110112).

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Authors and Affiliations

  1. Institute of Marine Drug and Food, Ocean University of China, Qingdao, 266003, P.R. China

    Xiaoke Hu & Xiaolu Jiang

  2. Department of Biology, Jackson State University, Jackson, Mississippi, 39217, U.S.A.

    Xiaoke Hu & Huey-min Hwang

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  1. Xiaoke Hu
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  2. Xiaolu Jiang
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  3. Huey-min Hwang
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Correspondence to Xiaoke Hu.

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Hu, X., Jiang, X. & Hwang, Hm. Purification and Characterization of an Alginate Lyase from Marine Bacterium Vibrio sp. Mutant Strain 510-64. Curr Microbiol 53, 135–140 (2006). https://doi.org/10.1007/s00284-005-0347-9

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  • DOI: https://doi.org/10.1007/s00284-005-0347-9

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