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Isolation and Characterization of Antifungal Substances from Burkholderia sp. Culture Broth

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Abstract

A new antagonistic Burkholderia strain, designated MP-1 and producing antifungal activities against various filamentous plant pathogenic fungi, was isolated from the rhizoshere in the Naju area. Cultural characteristic studies strongly suggested that this strain belongs to the genus Burkholderia. The nucleotide sequence of the 16S rRNA gene (1491 pb) of strain MP-1 exhibited close similarity (99% to 100%) with other Burkholderia 16S rRNA genes. Extraction of fermentation broth of Burkholderia sp. MP-1 and various separations and purification steps led to isolation of four pure active molecules. The chemical structure of these four compounds—named phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid, and 4-hydroxyphenylacetate methyl ester—was established on the basis on their gas chromatography–electron impact–mass spectrometry (GC-EI-MS) and trimethylsilation GC-EI-MS data. The four isolated compounds inhibited filamentous fungal growth on potato dextrose agar medium supplemented with 100 mg/L of phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid and 4-hydroxyphenylacetate methyl ester individually.

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Acknowledgments

This study was supported by a grant from the BioGreen 21 program, Rural Development Administration, and the Environmental-Friendly Agriculture Research Program (2005), Ministry of Agriculture, Republic of Korea.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Division of Applied Bioscience and Biotechnology and Institute of Agricultural Science and Technology, Environmental-Friendly Agriculture Research Center, Chonnam National University, 300, Yong-Bong Dong, Buk-Ku, Gwangju, 500-757, South Korea

    Sopheareth Mao, Hoon Hwangbo, Yong-Woong Kim, Ro-Dong Park & Kil-Yong Kim

  2. Department of Food Science and Technology, Division of Applied Bioscience and Biotechnology and Institute of Agricultural Science and Technology, Chonman National University, Gwangju, 500-757, Korea

    Seung-Je Lee & Keun-Hyung Park

  3. Department of Environmental Engineering, Gwangju University, Gwangju, 503-703, Korea

    Gyu-Suk Cha

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  1. Sopheareth Mao
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Correspondence to Kil-Yong Kim.

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Mao, S., Lee, SJ., Hwangbo, H. et al. Isolation and Characterization of Antifungal Substances from Burkholderia sp. Culture Broth. Curr Microbiol 53, 358–364 (2006). https://doi.org/10.1007/s00284-005-0333-2

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