Abstract
We isolated bacteriocin-producing Lactococcus lactis subsp. lactis from Kimchi. The bacteriocin inhibited strains of Clostridium perfringens, C. difficile, Listeria monocytogenes, vancomycin-resistant Enterococcus, and one out of four methicillin-resistant Staphylococcus aureus strains, as well as some closely related lactic acid bacteria. In tricine-SDS-PAGE, the bacteriocin migrated with an apparent molecular weight of about 4 kDa to the same location as nisin A and crude nisin Z. The gene encoding this bacteriocin was found to be identical to that of nisin Z with direct PCR sequence methods. The inhibitory activity was stable against heat and pH, but it was lost at 100°C for 1 h and at 121°C for 15 min. The bacteriocin was inactivated by proteolytic enzymes, but was not affected by lysozyme, lipase, catalase, or β-glucosidase. There were some differences in characteristics from those of nisins described previously.
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Received: 21 June 2002 / Accepted: 22 July 2002
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Park, SH., Itoh, K., Kikuchi, E. et al. Identification and Characteristics of Nisin Z-Producing Lactococcus lactis subsp. lactis Isolated from Kimchi. Curr Microbiol 46, 0385–0388 (2003). https://doi.org/10.1007/s00284-002-3898-z
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DOI: https://doi.org/10.1007/s00284-002-3898-z