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In Vivo Evaluation of a New Embolic Spherical Particle (HepaSphere) in a Kidney Animal Model

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Abstract

HepaSphere is a new spherical embolic material developed in a dry state that absorbs fluids and adapts to the vessel wall, leaving no space between the particle and the arterial wall. The aim of this study was to elucidate the final in vivo size, deformation, final location, and main properties of the particles when reconstituted with two different contrast media (Iodixanol and Ioxaglate) in an animal model. Two sizes of “dry-state” particles (50–100 and 150–200 μm) were reconstituted using both ionic and nonionic contrast media. The mixture was used to partly embolize both kidneys in an animal model (14 pigs). The animals were sacrificed 4 weeks after the procedure and the samples processed. The final size of the particles was 230.2 ± 62.5 μm for the 50- to 100-μm dry-state particles and 314.4 ± 71 μm for the 150- to 200-μm dry-state particles. When the contrast medium (ionic versus nonionic) used for the reconstitution was studied to compare (Student’s t-test) the final size of the particles, no differences were found (p > 0.05). The mean in vivo deformation for HepaSphere was 17.1% ± 12.3%. No differences (p > 0.05) were found in the deformation of the particle regarding the dry-state size or the contrast medium (Mann-Whitney test). We conclude that HepaSphere is stable, occludes perfectly, and morphologically adapts to the vessel lumen of the arteries embolized. There is no recanalization of the arteries 4 weeks after embolization. Its final in vivo size is predictable and the particle has the same properties in terms of size and deformation with the two different contrast media (Iodixanol and Ioxaglate).

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Acknowledgment

This study was supported by a grant from the Sociedad Española de Radiología Médica (SERAM).

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Authors and Affiliations

  1. Department of Radiology, Clínica Universitaria Navarra, Universidad de Navarra, Avda Pio XII 36, Pamplona, 31008, Navarra, Spain

    Esther de Luis, Jose I. Bilbao & Antonio Martínez-Cuesta

  2. Animal Pathology Department, Veterinary Faculty, Universidad de Zaragoza, C/Miguel Servet 177, Zaragoza, 50013, Spain

    Jose A. García Jalón de Ciércoles & Alba de Martino Rodríguez

  3. Pathology Department, Clínica Universitaria Navarra, Universidad de Navarra, Avda Pio XII 36, Pamplona, 31008, Navarra, Spain

    María D. Lozano

Authors
  1. Esther de Luis
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  2. Jose I. Bilbao
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  3. Jose A. García Jalón de Ciércoles
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  4. Antonio Martínez-Cuesta
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  5. Alba de Martino Rodríguez
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  6. María D. Lozano
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Correspondence to Esther de Luis.

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de Luis, E., Bilbao, J.I., de Ciércoles, J.A.G.J. et al. In Vivo Evaluation of a New Embolic Spherical Particle (HepaSphere) in a Kidney Animal Model. Cardiovasc Intervent Radiol 31, 367–376 (2008). https://doi.org/10.1007/s00270-007-9240-1

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  • DOI: https://doi.org/10.1007/s00270-007-9240-1

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