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Effectiveness of Topical Conditioned Medium of Stem Cells in Facial Skin Nonsurgical Resurfacing Modalities for Antiaging: Systematic Review and Meta-Analysis of Randomized Controlled Trials

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Abstract

Facial skin nonsurgical resurfacing modalities, including laser, chemical peeling, and microneedling, have become common due to increasing public concern about skin aging. The potential effect of stem cell conditioned medium (CM) for antiaging has been reported in recent years, and such medium may be able to improve the efficacy of resurfacing modalities. This study investigated the efficacy of topical CM combined with resurfacing in comparison with resurfacing alone. We searched the PubMed, Embase, and Cochrane Library databases for randomized controlled trials (RCTs). We used the Cochrane risk-of-bias tool (version 2) to assess the risk of bias of the included studies and Review Manager (version 5.4) for data analysis. Means and standard deviations of outcomes, namely wrinkle, pigmentation, pore, and overall improvement, were extracted. After screening, we included five RCTs in the analysis, four of which were quantitatively analyzed. The result revealed that stem cell CM significantly reduced wrinkles (P = 0.0006), pigmentation (P = 0.004), and pores (P = 0.01) and improved overall skin condition (P < 0.0001). In summary, we suggest that stem cell CM is a safe treatment that can enhance the efficacy of facial skin nonsurgical resurfacing modalities.

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References

  1. Lee H, Hong Y, Kim M (2021) Structural and functional changes and possible molecular mechanisms in aged skin. Int J Mol Sci. https://doi.org/10.3390/ijms222212489

    Article  PubMed  PubMed Central  Google Scholar 

  2. Kohl E, Steinbauer J, Landthaler M, Szeimies RM (2011) Skin ageing. J Eur Acad Dermatol Venereol 25(8):873–884. https://doi.org/10.1111/j.1468-3083.2010.03963.x

    Article  CAS  PubMed  Google Scholar 

  3. Devgan L, Singh P, Durairaj K (2019) Minimally invasive facial cosmetic procedures. Otolaryngol Clin North Am 52(3):443–459. https://doi.org/10.1016/j.otc.2019.02.013

    Article  PubMed  Google Scholar 

  4. Bhargava S, Goldust M, Singer H, Negbenebor N, Kroumpouzos G (2022) Evaluating resurfacing modalities in aesthetics. Clin Dermatol 40(3):274–282. https://doi.org/10.1016/j.clindermatol.2021.01.019

    Article  PubMed  Google Scholar 

  5. Goldberg DJ (2003) Lasers for facial rejuvenation. Am J Clin Dermatol 4(4):225–234. https://doi.org/10.2165/00128071-200304040-00002

    Article  PubMed  Google Scholar 

  6. Lee JC, Daniels MA, Roth MZ (2016) Mesotherapy, microneedling, and chemical peels. Clin Plast Surg 43(3):583–595. https://doi.org/10.1016/j.cps.2016.03.004

    Article  PubMed  Google Scholar 

  7. Lee KC, Wambier CG, Soon SL, Sterling JB, Landau M, Rullan P, Brody HJ (2019) Basic chemical peeling: superficial and medium-depth peels. J Am Acad Dermatol 81(2):313–324. https://doi.org/10.1016/j.jaad.2018.10.079

    Article  PubMed  Google Scholar 

  8. Lo KC, Chuang WW, Lamb DJ (2003) Stem cell research: the facts, the myths and the promises. J Urol 170(6 Pt 1):2453–2458. https://doi.org/10.1097/01.ju.0000087170.97532.ff

    Article  PubMed  Google Scholar 

  9. Damayanti RH, Rusdiana T, Wathoni N (2021) Mesenchymal stem cell secretome for dermatology application: a review. Clin Cosmet Investig Dermatol 14:1401–1412. https://doi.org/10.2147/ccid.S331044

    Article  PubMed  PubMed Central  Google Scholar 

  10. Park SR, Kim JW, Jun HS, Roh JY, Lee HY, Hong IS (2018) Stem cell secretome and its effect on cellular mechanisms relevant to wound healing. Mol Ther 26(2):606–617. https://doi.org/10.1016/j.ymthe.2017.09.023

    Article  CAS  PubMed  Google Scholar 

  11. Robert AW, Azevedo Gomes F, Rode MP, Marques da Silva M, Veleirinho M, Maraschin M, Stimamiglio MA (2019) The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells. J Tissue Eng 10:2041731419833391. https://doi.org/10.1177/2041731419833391

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, Moher D (2021) The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ 372:n71. https://doi.org/10.1136/bmj.n71

    Article  PubMed  PubMed Central  Google Scholar 

  13. Sterne JAC, Savović J, Page MJ, Elbers RG, Blencowe NS, Boutron I, Higgins JPT (2019) RoB 2: a revised tool for assessing risk of bias in randomised trials. BMJ 366:l4898. https://doi.org/10.1136/bmj.l4898

    Article  PubMed  Google Scholar 

  14. Seo KY, Kim DH, Lee SE, Yoon MS, Lee HJ (2013) Skin rejuvenation by microneedle fractional radiofrequency and a human stem cell conditioned medium in Asian skin: a randomized controlled investigator blinded split-face study. J Cosmet Laser Ther 15(1):25–33. https://doi.org/10.3109/14764172.2012.748201

    Article  PubMed  Google Scholar 

  15. Zhou BR, Zhang T, Bin Jameel AA, Xu Y, Xu Y, Guo SL, Luo D (2016) The efficacy of conditioned media of adipose-derived stem cells combined with ablative carbon dioxide fractional resurfacing for atrophic acne scars and skin rejuvenation. J Cosmet Laser Ther 18(3):138–148. https://doi.org/10.3109/14764172.2015.1114638

    Article  PubMed  Google Scholar 

  16. El-Domyati M, Moftah NH, Nasif GA, Ameen SW, Ibrahim MR, Ragaie MH (2020) Facial rejuvenation using stem cell conditioned media combined with skin needling: A split-face comparative study. J Cosmet Dermatol 19(9):2404–2410. https://doi.org/10.1111/jocd.13594

    Article  PubMed  Google Scholar 

  17. Hoss E, Kollipara R, Alhaddad M, Boen M, Goldman MP (2020) Red deer umbilical cord-derived stem cell conditioned media combined with ablative resurfacing of the face. J Drugs Dermatol 19(11):1044–1048. https://doi.org/10.36849/jdd.2020.5246

    Article  PubMed  Google Scholar 

  18. Lee HJ, Lee EG, Kang S, Sung JH, Chung HM, Kim DH (2014) Efficacy of microneedling plus human stem cell conditioned medium for skin rejuvenation: a randomized, controlled, blinded split-face study. Ann Dermatol 26(5):584–591. https://doi.org/10.5021/ad.2014.26.5.584

    Article  PubMed  PubMed Central  Google Scholar 

  19. Prakoeswa CRS, Pratiwi FD, Herwanto N, Citrashanty I, Indramaya DM, Murtiastutik D, Rantam FA (2019) The effects of amniotic membrane stem cell-conditioned medium on photoaging. J Dermatolog Treat 30(5):478–482. https://doi.org/10.1080/09546634.2018.1530438

    Article  CAS  PubMed  Google Scholar 

  20. Wang X, Shu X, Huo W, Zou L, Li L (2018) Efficacy of protein extracts from medium of Adipose-derived stem cells via microneedles on Asian skin. J Cosmet Laser Ther 20(4):237–244. https://doi.org/10.1080/14764172.2017.1400171

    Article  PubMed  Google Scholar 

  21. Badran KW, Nabili V (2018) Lasers, microneedling, and platelet-rich plasma for skin rejuvenation and repair. Facial Plast Surg Clin North Am 26(4):455–468. https://doi.org/10.1016/j.fsc.2018.06.007

    Article  PubMed  Google Scholar 

  22. Kim WS, Park BS, Park SH, Kim HK, Sung JH (2009) Antiwrinkle effect of adipose-derived stem cell: activation of dermal fibroblast by secretory factors. J Dermatol Sci 53(2):96–102. https://doi.org/10.1016/j.jdermsci.2008.08.007

    Article  CAS  PubMed  Google Scholar 

  23. Kim WS, Park BS, Kim HK, Park JS, Kim KJ, Choi JS, Sung JH (2008) Evidence supporting antioxidant action of adipose-derived stem cells: protection of human dermal fibroblasts from oxidative stress. J Dermatol Sci 49(2):133–142. https://doi.org/10.1016/j.jdermsci.2007.08.004

    Article  CAS  PubMed  Google Scholar 

  24. Liu Q, Luo Z, He S, Peng X, Xiong S, Wang Y, Gao BZ (2013) Conditioned serum-free medium from umbilical cord mesenchymal stem cells has anti-photoaging properties. Biotechnol Lett 35(10):1707–1714. https://doi.org/10.1007/s10529-013-1242-2

    Article  CAS  PubMed  Google Scholar 

  25. Abdel-Maguid EM, Awad SM, Hassan YS, El-Mokhtar MA, El-Deek HE, Mekkawy MM (2021) Efficacy of stem cell-conditioned medium vs. platelet-rich plasma as an adjuvant to ablative fractional CO(2) laser resurfacing for atrophic post-acne scars: a split-face clinical trial. J Dermatolog Treat 32(2):242–249. https://doi.org/10.1080/09546634.2019.1630701

    Article  CAS  PubMed  Google Scholar 

  26. El-Domyati M, Moftah NH, Nasif GA, Ragaie MH, Ibrahim MR, Ameen SW (2019) Amniotic fluid-derived mesenchymal stem cell products combined with microneedling for acne scars: a split-face clinical, histological, and histometric study. J Cosmet Dermatol. https://doi.org/10.1111/jocd.13039

    Article  PubMed  Google Scholar 

  27. Putri WE, Endaryanto A, Rantam FA, Prakoeswa CRS (2021) Mesenchymal stem cells-conditioned medium (Secretome) in skin aging: a systematic review. Int J Pharm Res 13(2):613–635. https://doi.org/10.31838/ijpr/2021.13.02.020

    Article  Google Scholar 

  28. Montero-Vilchez T, Sierra-Sánchez Á, Sanchez-Diaz M, Quiñones-Vico MI, Sanabria-de-la-Torre R, Martinez-Lopez A, Arias-Santiago S (2021) Mesenchymal stromal cell-conditioned medium for skin diseases: a systematic review. Front Cell Dev Biol 9:654210. https://doi.org/10.3389/fcell.2021.654210

    Article  PubMed  PubMed Central  Google Scholar 

  29. Sohn SJ, Yu JM, Lee EY, Nam YJ, Kim J, Kang S, Kang S (2018) Anti-aging properties of conditioned media of epidermal progenitor cells derived from mesenchymal stem cells. Dermatol Ther (Heidelb) 8(2):229–244. https://doi.org/10.1007/s13555-018-0229-2

    Article  PubMed  Google Scholar 

  30. Kim YJ, Seo DH, Lee SH, Lee SH, An GH, Ahn HJ, Kang KS (2018) Conditioned media from human umbilical cord blood-derived mesenchymal stem cells stimulate rejuvenation function in human skin. Biochem Biophys Rep 16:96–102. https://doi.org/10.1016/j.bbrep.2018.10.007

    Article  PubMed  PubMed Central  Google Scholar 

  31. Vashi NA, Wirya SA, Inyang M, Kundu RV (2017) Facial hyperpigmentation in skin of color: special considerations and treatment. Am J Clin Dermatol 18(2):215–230. https://doi.org/10.1007/s40257-016-0239-8

    Article  PubMed  Google Scholar 

  32. Solano F (2020) Photoprotection and skin pigmentation: melanin-related molecules and some other new agents obtained from natural sources. Molecules. https://doi.org/10.3390/molecules25071537

    Article  PubMed  PubMed Central  Google Scholar 

  33. Kim WS, Park SH, Ahn SJ, Kim HK, Park JS, Lee GY, Sung JH (2008) Whitening effect of adipose-derived stem cells: a critical role of TGF-beta 1. Biol Pharm Bull 31(4):606–610. https://doi.org/10.1248/bpb.31.606

    Article  CAS  PubMed  Google Scholar 

  34. Kim DW, Jeon BJ, Hwang NH, Kim MS, Park SH, Dhong ES, Lee BI (2014) Adipose-derived stem cells inhibit epidermal melanocytes through an interleukin-6-mediated mechanism. Plast Reconstr Surg 134(3):470–480. https://doi.org/10.1097/prs.0000000000000431

    Article  CAS  PubMed  Google Scholar 

  35. Xu Y, Guo S, Wei C, Li H, Chen L, Yin C, Zhang C (2016) The Comparison of adipose stem cell and placental stem cell in secretion characteristics and in facial antiaging. Stem Cells Int 2016:7315830. https://doi.org/10.1155/2016/7315830

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Kim BY, Choi JW, Park KC, Youn SW (2013) Sebum, acne, skin elasticity, and gender difference — which is the major influencing factor for facial pores? Skin Res Technol 19(1):e45-53. https://doi.org/10.1111/j.1600-0846.2011.00605.x

    Article  CAS  PubMed  Google Scholar 

  37. Lee SJ, Seok J, Jeong SY, Park KY, Li K, Seo SJ (2016) Facial pores: definition, causes, and treatment options. Dermatol Surg 42(3):277–285. https://doi.org/10.1097/dss.0000000000000657

    Article  CAS  PubMed  Google Scholar 

  38. Park CS, Park JH, Kim CR, Lee JH (2021) Objective analysis of volume restoration in atrophic acne scars and skin pores: a split study using human stem cell-conditioned media. J Dermatolog Treat 32(1):73–77. https://doi.org/10.1080/09546634.2019.1628915

    Article  CAS  PubMed  Google Scholar 

  39. Lee SH (2020) Effects of human endothelial progenitor cell and its conditioned medium on oocyte development and subsequent embryo development. Int J Mol Sci. https://doi.org/10.3390/ijms21217983

    Article  PubMed  PubMed Central  Google Scholar 

  40. Kim CH, Kim DH, Oh SH, Song SY (2015) Human embryonic stem cell-derived endothelial precursor cell conditioned medium reduces the thickness of the capsule around silicone implants in rats. Ann Plast Surg 75(3):348–352. https://doi.org/10.1097/sap.0000000000000123

    Article  CAS  PubMed  Google Scholar 

  41. Joseph A, Baiju I, Bhat IA, Pandey S, Bharti M, Verma M, Taru Sharma G (2020) Mesenchymal stem cell-conditioned media: a novel alternative of stem cell therapy for quality wound healing. J Cell Physiol 235(7–8):5555–5569. https://doi.org/10.1002/jcp.29486

    Article  CAS  PubMed  Google Scholar 

  42. Bhardwaj R, Ansari MM, Parmar MS, Chandra V, Sharma GT (2016) Stem cell conditioned media contains important growth factors and improves in vitro buffalo embryo production. Anim Biotechnol 27(2):118–125. https://doi.org/10.1080/10495398.2015.1118383

    Article  CAS  PubMed  Google Scholar 

  43. Vizoso FJ, Eiro N, Cid S, Schneider J, Perez-Fernandez R (2017) Mesenchymal stem cell secretome: toward cell-free therapeutic strategies in regenerative medicine. Int J Mol Sci. https://doi.org/10.3390/ijms18091852

    Article  PubMed  PubMed Central  Google Scholar 

  44. Takeuchi R, Katagiri W, Endo S, Kobayashi T (2019) Exosomes from conditioned media of bone marrow-derived mesenchymal stem cells promote bone regeneration by enhancing angiogenesis. PLoS ONE 14(11):e0225472. https://doi.org/10.1371/journal.pone.0225472

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. He C, Zheng S, Luo Y, Wang B (2018) Exosome theranostics: biology and translational medicine. Theranostics 8(1):237–255. https://doi.org/10.7150/thno.21945

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Xunian Z, Kalluri R (2020) Biology and therapeutic potential of mesenchymal stem cell-derived exosomes. Cancer Sci 111(9):3100–3110. https://doi.org/10.1111/cas.14563

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Zhang J, Li S, Li L, Li M, Guo C, Yao J, Mi S (2015) Exosome and exosomal microRNA: trafficking, sorting, and function. Genomics Proteomics Bioinform 13(1):17–24. https://doi.org/10.1016/j.gpb.2015.02.001

    Article  CAS  Google Scholar 

  48. Hu S, Li Z, Cores J, Huang K, Su T, Dinh PU, Cheng K (2019) Needle-free injection of exosomes derived from human dermal fibroblast spheroids ameliorates skin photoaging. ACS Nano 13(10):11273–11282. https://doi.org/10.1021/acsnano.9b04384

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Ha DH, Kim SD, Lee J, Kwon HH, Park GH, Yang SH, Yi YW (2020) Toxicological evaluation of exosomes derived from human adipose tissue-derived mesenchymal stem/stromal cells. Regul Toxicol Pharmacol 115:104686. https://doi.org/10.1016/j.yrtph.2020.104686

    Article  CAS  PubMed  Google Scholar 

  50. Xiong M, Zhang Q, Hu W, Zhao C, Lv W, Yi Y, Wu M (2020) Exosomes from adipose-derived stem cells: the emerging roles and applications in tissue regeneration of plastic and cosmetic surgery. Front Cell Dev Biol 8:574223. https://doi.org/10.3389/fcell.2020.574223

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This manuscript was edited by Wallace Academic Editing. This study was partially supported by Taipei Municipal Wanfang Hospital (managed by Taipei Medical University) (grant number: 111TMU-WFH-06).

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Author notes

  1. Ting-Jung Lin and Ya-Li Huang contributed equally to this work.

  2. Yi-No Kang and Chiehfeng Chen contributed equally to this work.

Authors and Affiliations

  1. School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

    Ting-Jung Lin

  2. Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, 250 Wuxing Street, Taipei, 110, Taiwan

    Ya-Li Huang & Chiehfeng Chen

  3. Cochrane Taiwan, Taipei Medical University, Taipei, Taiwan

    Yi-No Kang & Chiehfeng Chen

  4. Evidence-Based Medicine Center, Wan Fang Hospital, Medical University Hospital, Taipei, Taiwan

    Yi-No Kang & Chiehfeng Chen

  5. Research Center of Big Data and Meta-Analysis, Wan Fang Hospital, Taipei Medical University, 111 sec. 3 Xinlong Road, Taipei, 116, Taiwan

    Yi-No Kang

  6. Institute of Health Policy and Management, College of Public Health, National Taiwan University, Taipei, Taiwan

    Yi-No Kang

  7. Division of Plastic Surgery, Department of Surgery, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan

    Chiehfeng Chen

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Lin, TJ., Huang, YL., Kang, YN. et al. Effectiveness of Topical Conditioned Medium of Stem Cells in Facial Skin Nonsurgical Resurfacing Modalities for Antiaging: Systematic Review and Meta-Analysis of Randomized Controlled Trials. Aesth Plast Surg 47, 799–807 (2023). https://doi.org/10.1007/s00266-022-03168-z

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