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The Effects of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Neovascularization and Fat Graft Survival

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  • Basic Science/Experimental
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Abstract

Background

Adipose-derived stem cell (ADSCs)-assisted and platelet-rich plasma (PRP)-assisted lipofilling aim to enhance angiogenesis and cell proliferation and are promising techniques for lipofilling. This study aimed to compare the outcomes of ADSCs-assisted and PRP-assisted lipofilling.

Methods

Adipose tissue and human venous blood were obtained from women with early breast cancer. Human ADSCs were isolated and amplified in vitro. PRP was extracted through double centrifugation. The effect of PRP on ADSCs proliferation was evaluated. In the in vivo study, 1 ml of adipose tissue with saline (control group), PRP (PRP group), or ADSCs (ADSCs group) was injected subcutaneously into the dorsum of nude mice. At 2, 4, 8, and 12 weeks after injection, tissues were assessed for volume retention and ultrasound abnormality. For histological assessment, hematoxylin and eosin staining were performed.

Results

Cytokines in PRP and blood were comparable. Regarding the in vitro assay, PRP significantly improved ADSCs proliferation, and the effect was dose-dependent. Concerning the in vivo study, for each time point, ADSCs-assisted lipofilling showed superior volume maintenance. Similarly, the PRP group showed improved angiogenesis and fat survival, as compared with the control group. The angiogenic effect of PRP was inferior to that of ADSCs at most time points. No significant difference was observed at 12 weeks after lipofilling. Complication rates were comparable between the PRP group and ADSCs group.

Conclusions

PRP-assisted and ADSCs-assisted lipofilling can significantly improve the cosmetic results of grafted fat. PRP-assisted lipofilling, which is considered convenient and clinically available, is a promising technique to improve neovascularization and fat survival.

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Author information

Author notes

  1. Bing-Jun Xiong and Qiu-Wen Tan have contributed equally to this work.

Authors and Affiliations

  1. Department of Breast Surgery, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu, 610041, China

    Bing-Jun Xiong, Qiu-Wen Tan, Yu-Juan Chen, Di Zhang, Shen-Li Tang, Shu Zhang & Qing Lv

  2. Laboratory of Stem Cell and Tissue Engineering, West China Hospital, Sichuan University, Chengdu, 610041, China

    Qiu-Wen Tan & Yi Zhang

Authors
  1. Bing-Jun Xiong
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  3. Yu-Juan Chen
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Correspondence to Qing Lv.

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The authors declare that they have no conflicts of interest to disclose.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration Helsinki and its later amendments or comparable ethical standards. All applicable institutional and/or national guidelines for the care and use of animals were followed.

Informed Consent

Informed consent was obtained from all tissue donors.

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Xiong, BJ., Tan, QW., Chen, YJ. et al. The Effects of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Neovascularization and Fat Graft Survival. Aesth Plast Surg 42, 1–8 (2018). https://doi.org/10.1007/s00266-017-1062-1

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  • DOI: https://doi.org/10.1007/s00266-017-1062-1

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