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In Vitro N-Acetyl-l-Cysteine Promotes Proliferation and Suppresses Interleukin-8 Expression in Adipose-Derived Stem Cells

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Abstract

Adipose-derived stem cells (ADSCs) hold great promise for repair and regeneration of burn wounds by producing growth factors, but proinflammatory cytokines such as interleukin-8 (IL-8) released by ADSCs would potentially deepen the wound and inhibit healing. The reported research aimed to identify the effects of N-acetyl-l-cysteine (NAC) on the proliferation, death, and IL-8 production of ADSCs. In the presence or absence of NAC, ADSC proliferation was examined using a CCK-8 Kit, and cell death was evaluated by flow cytometry analysis. Subsequently, IL-8 mRNA expression was detected by reverse transcriptase-polymerase chain reaction and protein production by enzyme-linked immunoassay. Findings showed that cell proliferation in the NAC-treated group was a significant 1.53-fold greater than in the control group, that the apoptosis rate of ADSCs decreased by 55.4 % compared with the control group, and that the necrosis rate decreased by 48.8 %. Additionally, the IL-8 mRNA expression decreased to 46.2 ± 8.7 % that of the control group, and the IL-8 protein production decreased to 9.98 ± 0.57 %. The authors believe that NAC might be helpful in burn wound repair and regeneration by stimulating the proliferation of ADSCs, inhibiting cell death, and suppressing IL-8 production.

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Acknowledgments

This research was supported by a grant from the National Natural Science Foundation of China (Grant No. 81071579).

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Union Hospital, Huazhong Science and Technique University, Wuhan, 430022, Hubei, China

    Lingyun Xiong, Jiaming Sun, Jie Yang, Yanqing Yang, Yun Xia, Rongrong Wang & Xi Fu

  2. Department of Hand, Plastic, and Reconstructive Surgery, BG-Trauma Centre Ludwigshafen, University of Heidelberg, 67071, Ludwigshafen, Germany

    Christoph Hirche & Marcus Lehnhardt

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  1. Lingyun Xiong
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  2. Jiaming Sun
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  4. Jie Yang
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  9. Xi Fu
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Correspondence to Jiaming Sun.

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Xiong, L., Sun, J., Hirche, C. et al. In Vitro N-Acetyl-l-Cysteine Promotes Proliferation and Suppresses Interleukin-8 Expression in Adipose-Derived Stem Cells. Aesth Plast Surg 36, 1260–1265 (2012). https://doi.org/10.1007/s00266-012-9960-8

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  • DOI: https://doi.org/10.1007/s00266-012-9960-8

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