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Effects of continuous or intermittent low-magnitude high-frequency vibration on fracture healing in sheep

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Abstract

Purpose

Vibration therapy has been shown to improve fracture healing. In this study, we investigated the effects of continuous or different intermittent vibration regimens on fracture healing in sheep models on the basis of radiographs, mechanical, and biochemical testing.

Methods

The 63 right-hind metatarsals from 63 sheep (12-month-old) were osteotomized; followed by surgical fixation with a steel plate. Two weeks after the surgery, the sheep with right-hind metatarsal fractures were randomly divided into seven groups (n=9/group): control (no vibration treated), continuous vibration (CV), one, three, five, seven and 14-day intermittent vibration (named IV-1, -3, -5, -7, and -14, respectively) groups, which represented a cycle of the successive n-day vibration and successive n-day break. Vibration stimulation (F=35 Hz, a=0.25 g) lasted 15 minutes each treatment. After eight weeks with/without vibration treatment, the sheep were euthanized with intravenous anesthetic. The callus formation, mechanical properties, and biochemical compositions of fracture metatarsals were analyzed.

Results

In CV and IV-7 groups, X-ray images showed an increased callus volume around the fracture area. The bone elastic modulus and the concentrations of Ca, P, and Ca/P ratio of the area at 15 and 25 mm away from the fracture centerline were higher in CV and IV-7 groups compared with the other groups.

Conclusions

Our results demonstrate that both CV and IV-7 vibration patterns showed better improvement of fracture healing.

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Funding

This work was supported by the Program of the National Natural Science Foundation of China (Nos. 11432016) and the Program of the National Natural Science Foundation of China (Nos. 11602093).

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Authors and Affiliations

  1. Department of Cardiology and Echocardiography, First Hospital of Jilin University, Changchun, China

    Yanhui Li

  2. College of Mechanical Science and Engineering, Jilin University, Changchun, China

    Guozhao Liu & Junhao Lin

  3. Operating Theatre 1, The First Hospital of Jilin University, Changchun, China

    Jing Yu

  4. Department of Orthopedic Traumatology, First Hospital of Jilin University, Changchun, 130021, China

    Chen Li, Lei Tan, Baohui Hao, Chao Liu & Dong Zhu

  5. Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, China

    Xizheng Zhang

Authors
  1. Yanhui Li
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  2. Guozhao Liu
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  3. Jing Yu
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  4. Chen Li
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  5. Lei Tan
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  7. Chao Liu
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  8. Junhao Lin
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  9. Dong Zhu
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  10. Xizheng Zhang
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Corresponding author

Correspondence to Dong Zhu.

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Animals used in this article were approved by the Experimental Animal Management Guide of University of Jilin (Changchun, China).

Conflict of interest

The authors declare that they have no conflict of interest.

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Li, Y., Liu, G., Yu, J. et al. Effects of continuous or intermittent low-magnitude high-frequency vibration on fracture healing in sheep. International Orthopaedics (SICOT) 42, 939–946 (2018). https://doi.org/10.1007/s00264-018-3759-4

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  • DOI: https://doi.org/10.1007/s00264-018-3759-4

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