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Proteomic analysis of synovial fluid: insight into the pathogenesis of knee osteoarthritis

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Abstract

Purpose

We conducted a proteomic analysis of synovial fluid (SF) to identify differentially expressed proteins and analyse their correlation with osteoarthritis (OA) severity. Our primary purpose was to gain insight into the pathogenesis of OA.

Methods

SF samples were acquired from 12 knee OA patients and 12 non-OA controls (ten had a meniscus injury, two had a discoid meniscus and all exhibited intact articular cartilage) and sequentially subjected to two-dimensional electrophoresis (2-DE). The radiographic grading of knee OA was performed using the Kellgren-Lawrence criteria. Differentially expressed proteins were identified by matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS). Proteins of interest identified from SF were detected using an enzyme-linked immunosorbent assay (ELISA).

Results

A total of 31 protein spots showed significant differences (p < 0.05) between the sample groups; 25 of the 31 spots (80.6 %) were identified as proteins of interest. Among them 20 corresponded to up-regulation and five to down-regulation in OA samples. HLA-DR was one of the proteins up-regulated, which was confirmed by ELISA.

Conclusions

These observations have implications in delineating the protein expression underlying the pathogenesis of OA and facilitate further elucidation of molecular mechanisms involved in disease progression. Substantial alterations of the protein profile in SF may be associated with OA severity.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopaedics, General Hospital of PLA, No. 28 Fuxing Road, Haidian District, Beijing, People’s Republic of China, 100853

    Weixiong Liao, Zhongli Li, Yangmu Fu & Xiaowei Bai

  2. Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, People’s Republic of China

    Hongli Wang & Jie Wang

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  1. Weixiong Liao
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  2. Zhongli Li
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  3. Hongli Wang
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  4. Jie Wang
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Correspondence to Zhongli Li.

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Liao, W., Li, Z., Wang, H. et al. Proteomic analysis of synovial fluid: insight into the pathogenesis of knee osteoarthritis. International Orthopaedics (SICOT) 37, 1045–1053 (2013). https://doi.org/10.1007/s00264-012-1768-2

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  • DOI: https://doi.org/10.1007/s00264-012-1768-2

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