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Study on the effect of T-2 toxin combined with low nutrition diet on rat epiphyseal plate growth and development

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Abstract

The purpose of this study was to observe early lesions of rat epiphyseal plates and metaphysis caused by T-2 toxin and T-2 toxin combined with a low nutrition diet to determine possible pathogenic factors of Kashin-Beck disease (KBD). Ninety Wistar rats were divided into three groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T-2 toxin; and group C was fed with a low nutrition diet and T-2 toxin. The left knee specimens were collected, fixed in formaldehyde solution, stained by hematoxylin and eosin and Masson. After two weeks, the epiphyseal plate showed necrosis of chondrocytes in groups B and C. After four weeks, more obvious chondrocyte necrosis appeared. The positive rate of Lamellar necrosis in group C was significantly higher than that in groups B and A (P < 0.01). Metaphyseal trabecular bone showed sparse disorder and disruption in group C. T-2 toxin combined with a low nutrition diet could lead to more serious chondrocyte necrosis in the epiphyseal plate and disturb metaphyseal trabecular bone formation.

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References

  1. Pasteels JL, Liu FD, Hinsenkamp M, Rooze M, Mathieu F, Perlmutter N (2001) Histology of Kashin-Beck lesions. Int Orthop 25(3):151–153

    Article  CAS  PubMed  Google Scholar 

  2. Mo DX (1987) Pathology and selenium deficiency in Kashin-Beck Disease. In: Combs GF, Spallholz JE, Levander OA, Oldfield JE (eds) Selenium in biology and medicine, 3rd symposium. Van Nostrand Reinhold, New York, pp 924–933

    Google Scholar 

  3. Xiong G (2001) Diagnostic, clinical and radiological characteristics of Kashin-Beck disease in Shaanxi Province, PR China. Int Orthop 25:147–150

    Article  CAS  PubMed  Google Scholar 

  4. Chasseur C, Suetens C, Michel V, Mathieu F, Begaux F, Nolard N, Haubruge E (2001) A 4-year study of the mycological aspects of Kashin-Beck disease in Tibet. Int Orthop 25:154–158

    Article  CAS  PubMed  Google Scholar 

  5. Bai FY, Chen YD, Chen QT (1990) Investigation on the contamination fungi of grain in Tian-Shui Kashin-Beck disease areas. Chin J Cont Endem Dis 5:33–34

    Google Scholar 

  6. Haubruge E, Chasseur C, Debouck C, Begaux F, Suetens C, Mathieu F, Michel V, Gaspar C, Rooze M, Hinsenkamp M, Gillet P, Nollard N, Lognay G (2001) The prevalence of mycotoxins in Kashin-Beck disease. Int Orthop 25(3):159–162

    Article  CAS  PubMed  Google Scholar 

  7. Moreno-Reyes R et al (1998) Kashin-Beck osteoarthropathy in rural Tibet in relation to selenium and iodine status. N Engl J Med 3339:1112–1120

    Article  Google Scholar 

  8. Zhang WH, Neve J, Xu JP, Vanderpas J, Wang ZL (2001) Selenium, iodine and fungal contamination in Yulin District (People’s Republic of China) endemic for Kashin-Beck disease. Int Orthop 25:188–190

    Article  CAS  PubMed  Google Scholar 

  9. La Grange M, Mathieu F, Begaux F, Durand MC (2001) Kashin-Beck disease and drinking water in Central Tibet. Int Orthop 25(3):167–169

    Article  PubMed  Google Scholar 

  10. Yang C, Niu C, Bodo M, Gabriel E, Notohm H, Wolf E, Muller PK (1993) Fulvic acid supplementation and selenium deficiency disturb the structural integrity of mouse skeletal tissue. Biochem J 289:829–883

    CAS  PubMed  Google Scholar 

  11. Luo Y, Yoshizawa T, Yang J-S, Zhang S-Y, Zhang B-J (1992) A survey of occurrence of Fusarium mycotoxins in corn and wheat samples from Shanxi and Shanxi Provinces, China. Mycotoxin Res 8:85–91

    Article  Google Scholar 

  12. Xiong Y (1997) Toxic effect of T-2 toxin on articular cartilage in Chinese experimental mini-pig. Chin J Endem Dis 12:4–6

    Google Scholar 

  13. Chasseur C, Suetens C, Nolard N, Begaux F, Haubruge E (1997) Fungal contamination in barley and Kashin-Beck disease in Tibet. Lancet 350:1074

    Article  CAS  PubMed  Google Scholar 

  14. Wu YH, Andreas L, Jin ZD, Wang SM, Gerhard HS, Richard WB, Xia WL (2006) Holocene climate development on the central Tibetan Plateau: A sedimentary record from Cuoe Lake. Palaeogeogr Palaeoclimatol Palaeoecol 234:328–340

    Article  Google Scholar 

  15. Bai A, Zhai P, Liu X (2007) Climatology and trends of wet spells in China. Theor Appl Climatol 88:139–148

    Article  Google Scholar 

  16. Dang S, Yan H, Yamamoto S, Wang X, Zeng L (2004) Poor nutritional status of younger Tibetan children living at high altitudes. Eur J Clin Nutr 58:938–946

    Article  CAS  PubMed  Google Scholar 

  17. Mo DX (1982) KBD cartilage necrosis histopathology and clinical significance. Chin J Endemicol 1(2):116–120

    Google Scholar 

  18. Hinsenkamp M, Mathieu F, Claus W, Collard F, de Maertelaer V (2009) Effects of physical environment on the evolution of Kashin-Beck disease in Tibet. Int Orthop 33:1085–1088

    Article  CAS  PubMed  Google Scholar 

  19. Suetens C, Moreno-Reyes R, Chasseur C, Mathieu F, Begaux F, Haubruge E, Durand MC, Nève J, Vanderpas J (2001) Epidemiological support for a multifactorial aetiology of Kashin-Beck disease in Tibet. Int Orthop 25(3):180–187

    Article  CAS  PubMed  Google Scholar 

  20. Yang JB, Sun DJ, Wang G (1993) The observation on the culture of Fusarium isolated from the cereal produced in KBD areas causing cartilage necrosis of growth plate in broiler chickens. Chin J End Dis Prev 8(6):325–326

    Google Scholar 

  21. Rizzo A, Atroshi R, Hirvi T et al (1994) Protective effect of antioxidants against free radical–mediated lipid peroxidation induced by DON or T-2 toxin. J Vet Med A41:81–90

    Google Scholar 

  22. Vila B, Jaradat ZV, Marquardt RR, Frohlich AA (2002) Effect of T-2 toxin on in vivo lipid peroxidation and vitamin E status in mice. Food Chem Toxicol 40:479–486

    Article  CAS  PubMed  Google Scholar 

  23. Minervini F, Fornelli F, Lucivero G, Romano C, Visconti A (2005) T-2 toxin immunotoxicity on human B and T lymphoid cell lines. Toxicology 210:81–91

    Article  CAS  PubMed  Google Scholar 

  24. Eriksen GS, Pettersson H (2004) Toxicological evaluation of trichothecenes in animal feed. Anim Feed Sci Technol 114:205–239

    Article  CAS  Google Scholar 

  25. Shokri F, Heidari M, Gharagozloo S, Ghazi Khansari M (2000) In vitro inhibitory effects of antioxidants on cytotoxicity of T-2 toxin. Toxicology 146:171–176

    Article  CAS  PubMed  Google Scholar 

  26. Jaradat ZW, Borja V, Marquardt RR (2006) Adverse effects of T-2 toxin on chicken lymphocytes blastogenesis and its protection with vitamin E. Toxicology 225:90–96

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors wish to express their gratitude to Dr Xiu-qun Li and other teachers from the Tissue Engineering Lab, West China Hospital, Sichuan University, for providing experimental sites and technical assistance.

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Authors and Affiliations

  1. Orthopedic Department, West China Hospital, Sichuan University, No.17 Guo Xue Xiang, Chengdu, 610041, Sichuan, People’s Republic of China

    Yun-fen Yao, Peng-de Kang, Xing-bo Li, Jing Yang, Bing Shen, Zong-ke Zhou & Fu-xing Pei

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  1. Yun-fen Yao
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  2. Peng-de Kang
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  3. Xing-bo Li
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  4. Jing Yang
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  5. Bing Shen
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  6. Zong-ke Zhou
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  7. Fu-xing Pei
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Correspondence to Fu-xing Pei.

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A key project in The Eleventh Five-Year National Science and Technology Pillar Program, P.R. China (2007BA125B04).

The first two authors contributed equally to this work.

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Yao, Yf., Kang, Pd., Li, Xb. et al. Study on the effect of T-2 toxin combined with low nutrition diet on rat epiphyseal plate growth and development. International Orthopaedics (SICOT) 34, 1351–1356 (2010). https://doi.org/10.1007/s00264-010-0966-z

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  • DOI: https://doi.org/10.1007/s00264-010-0966-z

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