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Test–retest repeatability of myocardial blood flow and infarct size using 11C-acetate micro-PET imaging in mice

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Abstract

Purpose

Global and regional responses of absolute myocardial blood flow index (iMBF) are used as surrogate markers to assess response to therapies in coronary artery disease. In this study, we assessed the test–retest repeatability of iMBF imaging, and the accuracy of infarct sizing in mice using 11C-acetate PET.

Methods

11C-Acetate cardiac PET images were acquired in healthy controls, endothelial nitric oxide synthase (eNOS) knockout transgenic mice, and mice after myocardial infarction (MI) to estimate global and regional iMBF, and myocardial infarct size compared to 18F-FDG PET and ex-vivo histology results.

Results

Global test–retest iMBF values had good coefficients of repeatability (CR) in healthy mice, eNOS knockout mice and normally perfused regions in MI mice (CR = 1.6, 2.0 and 1.5 mL/min/g, respectively). Infarct size measured on 11C-acetate iMBF images was also repeatable (CR = 17 %) and showed a good correlation with the infarct sizes found on 18F-FDG PET and histopathology (r 2 > 0.77; p < 0.05).

Conclusion

11C-Acetate micro-PET assessment of iMBF and infarct size is repeatable and suitable for serial investigation of coronary artery disease progression and therapy.

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Acknowledgments

The authors thank Keegan Flowers for the synthesis of 11C-acetate, Christine Archer for technical support with micro-PET imaging, and Richard Seymour for his surgical contributions.

Compliance with ethical standards

ᅟ

Funding

This work was supported by a grant from the Canadian Institutes of Health Research (MOP-79311), an Ontario Research Fund grant for the Institute of Cardiovascular Therapeutics (ORF-RE-02-038), and a Heart and Stroke Foundation of Ontario grant for the Molecular and Functional Imaging Program (PRG-6242).

E.C. was supported by Ernest and Margaret Ford Endowed Research Funds.

R.S.B. is a Career Investigator supported by the HSFO and Tier 1 Chair in Cardiovascular Research supported by the University of Ottawa.

R.S.B. and R.dK. received grant funding from a government/industry research program (with GE Healthcare, Nordion, Lantheus Medical Imaging, and Jubilant DraxImage).

Conflicts of interest

E.C., M.M. and J.D.S. have no disclosures related to this work.

J.M.R., R.K. and R.dK. receive royalties from the sales of FlowQuant© software.

R.S.B. is a consultant for Lantheus Medical Imaging.

J.M.R., R.K., R.S.B. and R.dK. are consultants for Jubilant DraxImage.

Ethical approval

All experiments were conducted with the approval of the University of Ottawa Animal Care Committee and in accordance with the guidelines of the Canadian Council on Animal Care. All applicable international guidelines on the care and use of animals were followed.

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Authors and Affiliations

  1. National Cardiac PET Centre, University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, Ontario, K1Y 4W7, Canada

    Etienne Croteau, Jennifer M. Renaud, Matthew McDonald, Ran Klein, Jean N. DaSilva, Rob S. B. Beanlands & Robert A. deKemp

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  1. Etienne Croteau
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  2. Jennifer M. Renaud
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  3. Matthew McDonald
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  4. Ran Klein
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  7. Robert A. deKemp
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Corresponding author

Correspondence to Robert A. deKemp.

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Croteau, E., Renaud, J.M., McDonald, M. et al. Test–retest repeatability of myocardial blood flow and infarct size using 11C-acetate micro-PET imaging in mice. Eur J Nucl Med Mol Imaging 42, 1589–1600 (2015). https://doi.org/10.1007/s00259-015-3111-9

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  • DOI: https://doi.org/10.1007/s00259-015-3111-9

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