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68Ga-labelled DOTA-derivatised peptide ligands

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Abstract

68Ge/68Ga generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of 68Ge/68Ga generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the 68Ga eluate as well as labelling and purification steps for peptides, and can be used with different 68Ge/68Ga generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10–20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a “hot lab” by personnel with no previous experience in the preparation of PET radiopharmaceuticals.

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References

  1. Greene MW, Tucker WD. An improved gallium-68 cow. Int J Appl Radiat Isot 1961; 12:62–63.

    Article  CAS  Google Scholar 

  2. Hnatowich DJ. A method for the preparation and quality control of68Ga radiopharmaceuticals. J Nucl Med 1975; 16:764–768.

    CAS  PubMed  Google Scholar 

  3. Schuhmacher J, Maier-Borst W. A new68Ge/68Ga radioisotope generator system for production of 68Ga in dilute HCl. Int J Appl Radiat Isotopes 1981; 32:31–36.

    Article  CAS  Google Scholar 

  4. Ambe S.68Ge/68Ga generator with alpha-ferric oxide support. Appl Radiat Isot 1988; 39:49–51.

    Article  CAS  Google Scholar 

  5. Loch C, Maziere B, Comar D. A new generator for ionic Gallium-68. J Nucl Med 1980; 21:171–173.

    CAS  PubMed  Google Scholar 

  6. Kopecky P, Mudrova B.68Ge-68Ga generator for the production of 68Ga in ionic form. Int J Appl Radiat Isot 1974; 25:263–268.

    Article  CAS  Google Scholar 

  7. Lewis RE, Camin LL. Germanium-68/gallium-68 generator for the one step elution of ionic gallium-68. J Labelled Comp Radiopharm 1981; 18:164.

    Google Scholar 

  8. McElvany KD, Hopkins KT, Welch MJ. Comparison of68Ge/68Ga generator systems for radiopharmaceutical production. Int J Appl Radiat Isot 1984; 35:521–524.

    Article  CAS  Google Scholar 

  9. Nakayama M, Haratake M, Ono M, Koiso T, Harada K, Nakayama H, Yahara S, Ohmomo Y, Arano Y. A new68Ge/68Ga generator system using an organic polymer containing N-methylglucamine groups as adsorbent for 68Ge. Appl Radiat Isot 2003; 58:9–14.

    Article  CAS  PubMed  Google Scholar 

  10. De Jong M, Bakker WH, Krenning EP, Breeman WAP, van der Pluijm ME, Bernard BF, Visser TJ, Jermann E, Behe M, Powell P, Mäcke HR. Yttrium 90 and indium 111 labelling, receptor binding, and biodistribution of [DOTA-d-Phe1, Tyr3]octreotide, a promising somatostatin analogue for radionuclide therapy. Eur J Nucl Med 1997; 24:368–371.

    Article  PubMed  Google Scholar 

  11. Wild D, Schmitt JS, Ginj M, Maecke HR, Bernard BF, Krenning E, de Jong M, Wenger S, Reubi JC. DOTA-NOC, a high-affinity ligaand of somatostatin receptor subtypes 2, 3 and 5 for labelling with various radiometals. Eur J Nucl Med Mol Imaging 2003; 30:1338–1347.

    Article  CAS  PubMed  Google Scholar 

  12. Zhang H, Reubi JC, Schmitt, J, Waldherr C, Hinni K, Mueller J, Maecke HR. Synthesis and evaluation of bombesin analog modified with different linkers for radiopharmaceuticals. Eur J Nucl Med Mol Imaging 2002; 29:S91.

    Google Scholar 

  13. Heppler A. Auswirkungen von Metallkomplexgeometrien verschiedener Polyamino-Polycarboxylat-Chelatoren auf die Biologie und Pharmakologie von Metall-Peptid-Konjugaten. Dissertation,University Basel, 2000.

  14. Hofmann M, Maecke H, Börner AR, Weckesser E, Schöffski P, Oei ML, Schumacher J, Henze M, Heppler A, Meyer GJ, Knapp WH. Biokinetics and imaging with the somatostatin receptor PET radioligand68Ga -DOTATOC: preliminary data. Eur J Nucl Med 2001; 28:1751–1757.

    CAS  PubMed  Google Scholar 

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Acknowledgements

H.M. would like to thank the Swiss National Science Foundation for financial support.

The work was performed within the COST B12 action of the EEC.

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Authors and Affiliations

  1. Klinik für Nuklearmedizin, Medizinische Hochschule Hannover, Carl Neuberg Str.1, 30623, Hannover, Germany

    G.-J. Meyer, W. H. Knapp & M. Hofmann

  2. Universitätsspital Basel, Switzerland

    H. Mäcke

  3. Deutsches Krebsforschungszentrum, Heidelberg, Germany

    J. Schuhmacher

Authors
  1. G.-J. Meyer
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  2. H. Mäcke
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  3. J. Schuhmacher
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  4. W. H. Knapp
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  5. M. Hofmann
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Correspondence to G.-J. Meyer.

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Meyer, GJ., Mäcke, H., Schuhmacher, J. et al. 68Ga-labelled DOTA-derivatised peptide ligands. Eur J Nucl Med Mol Imaging 31, 1097–1104 (2004). https://doi.org/10.1007/s00259-004-1486-0

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  • DOI: https://doi.org/10.1007/s00259-004-1486-0

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