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Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli

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Abstract

The production of feruloyl esterase activity by Bacillus spp. and lactobacilli can be detected in an agar-plate assay. The assay involves the substitution of the main carbon source in specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains, were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli, Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The enzyme was released from harvested cells by sonication and showed pH and temperature optima of 6.5 and 30 °C respectively.

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Authors and Affiliations

  1. Food Science Division (Microbiology), Department of Agriculture for Northern Ireland, Newforge Lane, Belfast BT9 5PX, UK e-mail: John.Donaghy@dani.gov.uk Tel.: +44-1232-255531 Fax: +44-1232-668376, , , , , , IE

    J. Donaghy & P. F. Kelly

  2. Department of Food Science (Microbiology), The Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, UK, , , , , , GB

    A. M. McKay

Authors
  1. J. Donaghy
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  2. P. F. Kelly
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  3. A. M. McKay
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Received: 2 February 1998 / Received revision: 20 April 1998 / Accepted: 27 April 1998

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Donaghy, J., Kelly, P. & McKay, A. Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli. Appl Microbiol Biotechnol 50, 257–260 (1998). https://doi.org/10.1007/s002530051286

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  • DOI: https://doi.org/10.1007/s002530051286

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