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Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth

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Abstract

The influence of several parameters on the fermentative production of nisin Z by Lactococcus lactis IO-1 was studied. Considerable attention has been focused on the relationship between the primary metabolite production of bacteriocin and lactate and cell growth, which has so far not been clarified in detail. Production of nisin Z was optimal at 30°C and in the pH range 5.0–5.5. The addition of Ca2+ to the medium showed a stimulating effect on the production of nisin Z. A maximum activity of 3150 IU/ml was obtained during pH-controlled batch fermentation in the medium supplemented with 0.1 M CaCl2. It was about three times higher than that obtained under the optimal conditions for cell growth and lactic acid production.

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References

  • Araya T, Ishibashi N, Shimamura S (1992) Genetic evidence that Lactococcus lactis JCM7638 produces a mutated form of nisin. J Gen Appl Microbiol 38: 271–278

    Article  CAS  Google Scholar 

  • Biswas SR, Ray P, Johnson MC, Ray B (1991) Influence of growth conditions on the production of a bacteriocin, pediocin AcH, by Pediococcus acidilactici H. Appl Environ Microbiol 57: 1265–1267

    CAS  Google Scholar 

  • Buchman GW, Banerjee S, Hansen JN (1988) Structure, expression, and evolution of a gene encoding the precursor nisin, a small protein antibiotic. J Biol Chem 263: 16260–16266

    CAS  Google Scholar 

  • Daba H, Lacroix C, Huang J, Simard RE (1993) Influence of growth conditions on production and activity of mesenterocin 5 by a strain of Leuconostoc mesenteroides. Appl Microbiol Biotech-nol 39: 166–173

    CAS  Google Scholar 

  • De Vuyst L, Vandamme EJ (1991) Microbial manipulation of nisin biosynthesis and fermentation. In: Jung, G, Sahl, H-G (eds) Nisin and novel lantibiotics, ESCOM Science Publishers B.V., Leiden, pp 397–409

    Google Scholar 

  • De Vuyst L, Vandamme EJ (1992) Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. J Gen Microbiol 138: 571–578

    Google Scholar 

  • De Vuyst L, Vandamme EJ (1993) Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. lactis batch fermentations using a complex medium. Appl Microbiol Biotechnol 40: 17–22

    Article  Google Scholar 

  • Gross E, Morell JL (1971) The structure of nisin. J Am Chem Soc 93: 4634–4635

    Article  CAS  Google Scholar 

  • Henning S, Metz R, Hammes WP (1986) Studies on the mode of action of nisin. Int J Food Microbiol 3: 121–134

    Article  CAS  Google Scholar 

  • Hörner T, Zähner H, Kellner R, Günther J (1989) Fermentation and isolation of epidermin, a lanthionine containing polypeptide antibiotic from Staphylococcus epidermidis. Appl Microbiol Biotechnol 30: 219–225

    Article  Google Scholar 

  • Ishizaki A, Ohta T (1989) Batch culture kinetics of l-lactate fermentation employing Streptococcus sp. IO-1. J Ferment Bioeng 67: 46–51

    Article  CAS  Google Scholar 

  • Ishizaki A, Osajima K, Nakamura K, Kimura K, Hara T, Ezaki T (1990a) Biochemical characterization of Lactococcus lactis IO-1 whose optimal temperature is as high as 37°C. J Gen Appl Microbiol 36: 1–6

    Article  CAS  Google Scholar 

  • Ishizaki A, Hara T, Ezaki T (1990b) Lactococcus lactis IO-1 (JCM 7638), a new strain of homofermentative l-lactate producing coccus. In: Christiansen C, Munck L, Villadsen J (eds) 5th European Congress on Biotechnology. Proceedings 1, Nørharven A/S, Viborg, pp 306–309

    Google Scholar 

  • Ishizaki A, Ueda T, Tanaka K, Stanbury PF (1992a) l-Lactate production from xylose employing Lactococcus lactis IO-1. Biotechnol Lett 14: 599–604

    Article  CAS  Google Scholar 

  • Ishizaki A, Matsusaki H, Uryu K, Inui T, Kurematsu K, Ichii S, Hayami T, Tonokawa M, Koba Y (1992b) Biochemical properties of a new lantibiotic produced by Lactococcus lactis IO-1. J Fac Agric Kyushu Univ 37: 1–11

    CAS  Google Scholar 

  • Ishizaki A, Tamura M, Kimura K, Koba Y, Tonokawa M, Ezaki T (1992c) Characterization of newly isolated Bacillus subtilis C1 which is sensitive to antibiotic produced by lactic acid coccus. J Ferment Bioeng 74: 248–250

    Article  Google Scholar 

  • Ishizaki A, Ueda T, Tanaka K, Stanbury PF (1993) The kinetics of end-product inhibition of l-lactate production from xylose and glucose by Lactococcus lactis IO-1. Biotechnol Lett 15: 489–494

    Article  CAS  Google Scholar 

  • Jensen PR, Hammer K (1993) Minimal requirements for exponential growth of Lactococcus lactis. Appl Environ Microbiol 59: 4363–4366

    CAS  Google Scholar 

  • Jung G (1991) Lantibiotics: a survey. In: Jung G, Sahl H-G (eds) Nisin and novel lantibiotics. ESCOM, Leiden, pp 1–34

    Google Scholar 

  • Kaletta C, Entian K-D (1989) Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product. J Bacteriol 171: 1597–1601

    CAS  Google Scholar 

  • Matsusaki H, Endo N, Sonomoto K, Ishizaki A (1995) Purification and identification of a peptide antibiotic produced by Lactococcus lactis IO-1. J Fac Agric Kyushu Univ 40: 73–85

    CAS  Google Scholar 

  • Meer JRvan der, Polman J, Beerthuyzen MM, Siezen RJ, Kuipers OP, Vos WMde (1993) Characterization of the Lactococcus lactis nisin A operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J Bacteriol 175: 2578–2588

    Google Scholar 

  • Mulders JWM, Boerrigter IJ, Rollema HS, Siezen RJ, Vos WMde. (1991) Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. Eur J Biochem 201: 581–584

    Article  CAS  Google Scholar 

  • Parente E, Ricciardi A, Addario G (1994) Influence of pH on growth and bacteriocin production by Lactococcus lactis subsp. lactis 140NWC during batch fermentation. Appl Microbiol Biotechnol 41: 388–394

    CAS  Google Scholar 

  • Siezen RJ, Rollema HS, Kuipers OP, Vos WMde (1995) Homology modelling of the Lactococcus lactis leader peptidase NisP and its interaction with the precursor of the lantibiotic nisin. Protein Eng 8: 117–125

    Article  CAS  Google Scholar 

  • Vaughan EE, Daly C, Fitzgerald GF (1992) Identification and characterization of helveticin V-1829, a bacteriocin produced by Lactobacillus helveticus 1829. J Appl Bacteriol 73: 299–308

    CAS  Google Scholar 

  • Yang R, Johnson MC, Ray B (1992) Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Environ Microbiol 58: 3355–3359

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan

    H. Matsusaki, N. Endo, K. Sonomoto & A. Ishizaki

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  1. H. Matsusaki
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  2. N. Endo
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  3. K. Sonomoto
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  4. A. Ishizaki
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Matsusaki, H., Endo, N., Sonomoto, K. et al. Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth. Appl Microbiol Biotechnol 45, 36–40 (1996). https://doi.org/10.1007/s002530050645

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  • DOI: https://doi.org/10.1007/s002530050645

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