Summary
The relative contributions of lactate inhibition and the generation of sterile (undividing) cells to the low xylose utilisation rate of Lactococcus lactis IO-1 was investigated. The lactate inhibition constant of xylose grown cells was shown to be 9.3 times more than that of glucose grown cells. However, the sterile cell production rate and LDH inactivation rate of the xylose cultures were at least 10 times less than the glucose cultures. Thus, it is suggested that the slower substrate consumption rate in xylose medium is caused mainly by the large inhibition constant for the end product.
Similar content being viewed by others
References
Ishizaki, A., Osajima, K., Nakamura, K., Kimura, K., Hara, T., and Ezaki, T. (1990). J. Gen. Appl. Microbiol., 36, (1), 1–6.
Ishizaki, A., Ohta, T., and Kobayashi, G. (1992). J. Biotechnol., 24, (1), 85–107.
Ishizaki, A., Ueda, T., Tanaka, K., and Stanbury, P. F. (1992a). Biotechnology Letters, 14, (7), 599–604.
Ishizaki, A., and Kobayashi, G. (1990). J. Ferment. Bioeng., 70, (2), 139–140.
Pirt, S. J., (1975). Principles of Microbe and Cell Cultivation, pp 57–58, Oxford, Blackwell.
Roy, D., Goulet, J., and LeDuy, A. (1986). Appl. Microbiol. Biotechnol., 24, 206–213.
Tyree, R. W., Clausen, E. C., and Gaddy, J. L. (1990). Biotechnology Letters, 12, (1), 51–56.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ishizaki, A., Ueda, T., Tanaka, K. et al. The kinetics of end-product inhibition of l-lactate production from xylose and glucose by Lactococcus lactis IO-1. Biotechnol Lett 15, 489–494 (1993). https://doi.org/10.1007/BF00129324
Issue Date:
DOI: https://doi.org/10.1007/BF00129324