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Halomonas spp., as chassis for low-cost production of chemicals

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Abstract

Halomonas spp. are the well-studied platform organisms or chassis for next-generation industrial biotechnology (NGIB) due to their contamination-resistant nature combined with their fast growth property. Several Halomonas spp. have been studied regarding their genomic information and molecular engineering approaches. Halomonas spp., especially Halomonas bluephagenesis, have been engineered to produce various biopolyesters such as polyhydroxyalkanoates (PHA), proteins including surfactants and enzymes, small molecular compounds including amino acids and derivates, as well as organic acids. This paper reviews all the progress reported in the last 10 years regarding this robust microbial cell factory.

Key points

• Halomonas spp. are robust chassis for low-cost production of chemicals

• Genomic information of some Halomonas spp. has been revealed

• Molecular tools and approaches for Halomonas spp. have been developed

• Halomonas spp. are becoming more and more important for biotechnology

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Acknowledgements

We are grateful for the kind donation of pSEVA plasmids from Professor Victor de Lorenzo of Ctr Nacl Biotecnol CSIC/Spain.

Funding

This work was financially supported by grants from the Ministry of Science and Technology of China (Grant No. 2018YFA0900200), National Natural Science Foundation of China (Grant No. 21761132013; No. 31870859; No. 92068117), Tsinghua University-INDITEX Sustainable Development Fund (Grant No. TISD201907), and Center of Life Sciences of Tsinghua-Peking University. This project was also funded by the National Natural Science Foundation of China (Grant No. 31961133017; No. 31961133018; No. 31961133019). These grants are part of MIX-UP, a joint NSFC and EU H2020 collaboration. In Europe, MIX-UP has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement no. 870294.

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Authors and Affiliations

  1. MOE Key Laboratory for Industrial Biocatalysts, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

    Guo-Qiang Chen & Xu Zhang

  2. School of Life Sciences, Tsinghua University, Beijing, 100084, China

    Guo-Qiang Chen

  3. Tsinghua-Peking Center of Life Sciences, Beijing, 100084, China

    Guo-Qiang Chen & Xu Zhang

  4. Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China

    Guo-Qiang Chen

  5. PhaBuilder Biotechnol Co. Ltd. Shunyi District, Zhaoquan Ying, Beijing, 101309, China

    Xu Liu

  6. Peking University International Cancer Institute, Peking University Health Science Center, Peking University, Haidian District, Beijing, 100191, China

    Weiran Huang & Zhengwei Xie

  7. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Jing Han, Tong Xu, Ruchira Mitra & Cheng Zhou

  8. International College, University of Chinese Academy of Sciences, 100049, Beijing, People’s Republic of China

    Ruchira Mitra

  9. Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China

    Jing Zhang & Tao Chen

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  1. Guo-Qiang Chen
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  10. Jing Zhang
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Contributions

GQC designed, conceptualized, edited, and supervised the entire work. XZ, WH, JH, and JZ prepared the primary draft. XZ, ZX, CZ, and TC revised it and designed the figures. XL, TX, and RM prepared the tables. All authors read and approved the manuscript.

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Correspondence to Guo-Qiang Chen.

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Chen, GQ., Zhang, X., Liu, X. et al. Halomonas spp., as chassis for low-cost production of chemicals. Appl Microbiol Biotechnol 106, 6977–6992 (2022). https://doi.org/10.1007/s00253-022-12215-3

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