Abstract
As the core food crop of a bioregenerative life support system (BLSS), wheat is susceptible to pathogen infection due to the lack of effective microbial communities in the confined and isolated environment. Therefore, a thorough understanding of the dynamic changes in wheat rhizosphere fungi is of great significance for improving wheat production and ensuring the stability of the BLSS. In the current study, we collected samples of rhizosphere fungi in the four growth stages of wheat grown in the “Lunar Palace 365” experiment. We employed bioinformatics methods to analyze the samples’ species composition characteristics, community network characteristics, and FUNGuild function analysis. We found that the species composition of rhizosphere fungi in the wheat at the tillering stage changed greatly in the closed and isolated environment, while the species composition in the seedling, flowering, and mature stage were relatively stable. The results of the FUNGuild function analysis showed that the functions of rhizosphere fungi changed during wheat development. The rhizosphere fungal community was centered on Ascomycota, Mortierellomycota, and Chytridiomycota, and the community showed the characteristics of a “small world” arrangement. The stage of wheat seedlings is characterized by a greater abundance, diversity, and complexity of the network of interactions in the rhizosphere mycorrhiza community, while the tillering stage exhibited a greater clustering coefficient. Based on the changes in species composition, guild function regulation, and community structure differences of the wheat rhizosphere fungi in the BLSS, our study identified the critical fungal species during wheat development, providing a reference for ensuring the health and yield of plants in the BLSS system.
Key points
• The diversity, composition, FUNguild, and network structure of rhizosphere fungi were analyzed.
• Ascomycota, Mortierellomycota, and Chytridiomycota were the center of the rhizosphere fungal community network.
• The effects of different wheat developmental stages on the community composition, function, and network structure of rhizosphere fungi were examined.
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Data availability
The fungal 16S rRNA gene sequencing data reported in this paper have been deposited in the Genome Sequence Archive (Genomics, Proteomics & Bioinformatics 2017) in the National Genomics Data Center (Nucleic Acids Res 2020), Beijing Institute of Genomics (China National Center for Bioinformation), Chinese Academy of Sciences, under accession number(s) CRA004749 that are publicly accessible at https://bigd.big.ac.cn/gsa. All other data are available from the authors upon reasonable request.
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This work was financially supported by the Civil Aerospace Technology Advance Research Project (B0107) and the National Natural Science Foundation of China (31870852).
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YF, ZY, and HL conceived and designed the research. ZY conducted the experiments. RS analyzed the data. YF guided most experiments. RS and YF wrote the manuscript. All authors read and approved the manuscript.
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Sun, R., Yi, Z., Fu, Y. et al. Dynamic changes in rhizosphere fungi in different developmental stages of wheat in a confined and isolated environment. Appl Microbiol Biotechnol 106, 441–453 (2022). https://doi.org/10.1007/s00253-021-11698-w
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DOI: https://doi.org/10.1007/s00253-021-11698-w