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Metabolic engineering of Parageobacillus thermoglucosidasius for the efficient production of (2R, 3R)-butanediol

  • Biotechnological products and process engineering
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Abstract

High-temperature fermentation using thermophilic microorganisms may provide cost-effective processes for the industrial production of fuels and chemicals, due to decreased hygiene and cooling costs. In the present study, the genetically trackable thermophile Parageobacillus thermoglucosidasius DSM2542T was engineered to produce (2R, 3R)-butanediol (R-BDO), a valuable chemical with broad industrial applications. The R-BDO biosynthetic pathway was optimized by testing different combinations of pathway enzymes, with acetolactate synthase (AlsS) from Bacillus subtilis and acetolactate decarboxylase (AlsD) from Streptococcus thermophilus yielding the highest production in P. thermoglucosidasius DSM2542T. Following fermentation condition optimization, shake flask fermentation at 55 °C resulted in the production of 7.2 g/L R-BDO with ~ 72% theoretical yield. This study details the microbial production of R-BDO at the highest fermentation temperature reported to date and demonstrates that P. thermoglucosidasius DSM2542T is a promising cell factory for the production of fuels and chemicals using high-temperature fermentation.

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Funding

This work was supported by the Energy Biosciences Institute.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Jiewen Zhou, Jiazhang Lian & Christopher V. Rao

  2. Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd, 866 Moganshan Road, Hangzhou, 310011, China

    Jiewen Zhou

  3. College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China

    Jiazhang Lian

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  1. Jiewen Zhou
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  2. Jiazhang Lian
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  3. Christopher V. Rao
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Correspondence to Christopher V. Rao.

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Zhou, J., Lian, J. & Rao, C.V. Metabolic engineering of Parageobacillus thermoglucosidasius for the efficient production of (2R, 3R)-butanediol. Appl Microbiol Biotechnol 104, 4303–4311 (2020). https://doi.org/10.1007/s00253-020-10553-8

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  • DOI: https://doi.org/10.1007/s00253-020-10553-8

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