Abstract
Self-healing mechanisms are a promising solution to address the concrete cracking issue. Among the investigated self-healing strategies, the biotechnological approach is distinguished itself by inducing the most compatible material with concrete composition. In this method, the potent bacteria and nutrients are incorporated into the concrete matrix. Once cracking occurs, the bacteria will be activated, and the induced CaCO3 crystals will seal the concrete cracks. However, the effectiveness of a bio self-healing concrete strictly depends on the viability of bacteria. Therefore, it is required to protect the bacteria from the resulted shear forces caused by mixing and drying shrinkage of concrete. Due to the positive effects on mechanical properties and the high compatibility of metallic nanoparticles with concrete composition, for the first time, we propose 3-aminopropyltriethoxy silane-coated iron oxide nanoparticles (APTES-coated IONs) as a biocompatible carrier for Bacillus species. This study was aimed to investigate the effect of APTES-coated IONs on the bacterial viability and CaCO3 yield for future application in the concrete structures. The APTES-coated IONs were successfully synthesized and characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The results show that the presence of 100 μg/mL APTES-coated IONs could increase the bacterial viability. It was also found that the CaCO3-specific yield was significantly affected in the presence of APTES-coated IONs. The highest CaCO3-specific yield was achieved when the cells were decorated with 50 μg/mL of APTES-coated IONs. This study provides new insights for the application of APTES-coated IONs in designing bio self-healing strategies.
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References
Abdal Dayem A, Hossain MK, Lee SB, Kim K, Saha SK, Yang G-M, Choi HY, Cho S-G (2017) The role of reactive oxygen species (ROS) in the biological activities of metallic nanoparticles. Int J Mol Sci 18:120. https://doi.org/10.3390/ijms18010120
Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A (2012) Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. Int J Nanomedicine 7:6003–6009. https://doi.org/10.2147/IJN.S35347
Bang SS, Galinat JK, Ramakrishnan V (2001) Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enzym Microb Technol 28:404–409. https://doi.org/10.1016/S0141-0229(00)00348-3
Brunner TJ, Wick P, Manser P, Spohn P, Grass RN, Limbach LK, Bruinink A, Stark WJ (2006) In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos, silica, and the effect of particle solubility. Environ Sci Technol 40(14):4374–4381. https://doi.org/10.1021/es052069i
Chang T-P, Shih J-Y, Yang K-M, Hsiao T-C (2007) Material properties of Portland cement paste with nano-montmorillonite. J Mater Sci 42(17):7478–7487. https://doi.org/10.1007/s10853-006-1462-0
Chatterjee S, Bandyopadhyay A, Sarkar K (2011) Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application. J Nanobiotechnol 9(1):34. https://doi.org/10.1186/1477-3155-9-34
De Belie N (2016) Application of bacteria in concrete: a critical evaluation of the current status. RILEM Tech Lett 1:56–61. 10.21809/rilemtechlett.2016.14
Dias A, Hussain A, Marcos A, Roque A (2011) A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharides. Biotechnol Adv 29(1):142–155. https://doi.org/10.1016/j.biotechadv.2010.10.003
Dinali R, Ebrahiminezhad A, Manley-Harris M, Ghasemi Y, Berenjian A (2017a) The effect of iron oxide nanoparticles on Bacillus subtilis biofilm, growth and viability. Process Biochem 62:231–240
Dinali R, Ebrahiminezhad A, Manley-Harris M, Ghasemi Y, Berenjian A (2017b) Iron oxide nanoparticles in modern microbiology and biotechnology. Crit Rev Microbiol 43:493–507
Ebrahiminezhad A, Rasoul-Amini S, Kouhpayeh A, Davaran S, Barar J, Ghasemi Y (2015) Impacts of amine functionalized iron oxide nanoparticles on HepG2 cell line. Curr Nanosci 11:113–119
Ebrahiminezhad A, Varma V, Yang S, Berenjian A (2016) Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation. Appl Microbiol Biotechnol 100(1):173–180. https://doi.org/10.1007/s00253-015-6977-3
Jayapalan A, Lee B, Fredrich S, Kurtis K (2010) Influence of additions of anatase TiO2 nanoparticles on early-age properties of cement-based materials. Transp Res Theatr Rec 2141:41–46
Jo BW, Kim CH, Gh T, Park JB (2007) Characteristics of cement mortar with nano-SiO2 particles. Constr Build Mater 21(6):1351–1355. https://doi.org/10.1016/j.conbuildmat.2005.12.020
Konsta-Gdoutos MS, Metaxa ZS, Shah SP (2010) Highly dispersed carbon nanotube reinforced cement based materials. Cem Concr Res 40(7):1052–1059. https://doi.org/10.1016/j.cemconres.2010.02.015
Kourkoutas Y, Bekatorou A, Banat IM, Marchant R, Koutinas AA (2004) Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiol 21:377–397. https://doi.org/10.1016/j.fm.2003.10.005
Martin ST, Morrison CL, Hoffmann MR (1994) Photochemical mechanism of size-quantized vanadium-doped TiO2 particles. J Phys Chem 98(51):13695–13704. https://doi.org/10.1021/j100102a041
Qing Y, Zenan Z, Deyu K, Rongshen C (2007) Influence of nano-SiO2 addition on properties of hardened cement paste as compared with silica fume. Constr Build Mater 21(3):539–545. https://doi.org/10.1016/j.conbuildmat.2005.09.001
Santra S, Tapec R, Theodoropoulou N, Dobson J, Hebard A, Tan W (2001) Synthesis and characterization of silica-coated iron oxide nanoparticles in microemulsion: the effect of nonionic surfactants. Langmuir 17(10):2900–2906. https://doi.org/10.1021/la0008636
Sato T, Diallo F (2010) Seeding effect of nano-CaCO3 on the hydration of tricalcium silicate. Transp Res Theatr Rec 2141:61–67
Seifan M, Samani AK, Burgess JJ, Berenjian A (2016a) The effectiveness of microbial crack treatment in self healing concrete. In: Berenjian A, Jafarizadeh-Malmiri H, Song Y (eds) High value processing technologies. Nova Science publishers, New York, pp 97–124
Seifan M, Samani AK, Berenjian A (2016b) Bioconcrete: next generation of self-healing concrete. Appl Microbiol Biotechnol 100(6):2591–2602. https://doi.org/10.1007/s00253-016-7316-z
Seifan M, Samani AK, Berenjian A (2016c) Induced calcium carbonate precipitation using Bacillus species. Appl Microbiol Biotechnol 100(23):9895–9906. https://doi.org/10.1007/s00253-016-7701-7
Seifan M, Samani AK, Berenjian A (2017) New insights into the role of pH and aeration in the bacterial production of calcium carbonate (CaCO3). Appl Microbiol Biotechnol 101(8):3131–3142. https://doi.org/10.1007/s00253-017-8109-8
Sies H (1997) Oxidative stress: oxidants and antioxidants. Exp Physio 82(2):291–295. https://doi.org/10.1113/expphysiol.1997.sp004024
Sikora P, Horszczaruk E, Cendrowski K, Mijowska E (2016) The influence of nano-Fe3O4 on the microstructure and mechanical properties of cementitious composites. Nanoscale Res Lett 11(1):182. https://doi.org/10.1186/s11671-016-1401-1
Sundaram PA, Augustine R, Kannan M (2012) Extracellular biosynthesis of iron oxide nanoparticles by Bacillus subtilis strains isolated from rhizosphere soil. Biotechnol Bioprocess Eng 17(4):835–840. https://doi.org/10.1007/s12257-011-0582-9
Vučak M, Pons MN, Perić J, Vivier H (1998) Effect of precipitation conditions on the morphology of calcium carbonate: quantification of crystal shapes using image analysis. Powder Technol 97(1):1–5. https://doi.org/10.1016/S0032-5910(97)03375-5
Wang J, Van Tittelboom K, De Belie N, Verstraete W (2012a) Use of silica gel or polyurethane immobilized bacteria for self-healing concrete. Constr Build Mater 26(1):532–540. https://doi.org/10.1016/j.conbuildmat.2011.06.054
Wang JY, De Belie N, Verstraete W (2012b) Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. J Ind Microbiol Biotechnol 39(4):567–577. https://doi.org/10.1007/s10295-011-1037-1
Wang JY, Soens H, Verstraete W, De Belie N (2014a) Self-healing concrete by use of microencapsulated bacterial spores. Cem Concr Res 56:139–152. https://doi.org/10.1016/j.cemconres.2013.11.009
Wang JY, Snoeck D, Van Vlierberghe S, Verstraete W, De Belie N (2014b) Application of hydrogel encapsulated carbonate precipitating bacteria for approaching a realistic self-healing in concrete. Constr Build Mater 68:110–119. https://doi.org/10.1016/j.conbuildmat.2014.06.018
Wiktor V, Jonkers HM (2011) Quantification of crack-healing in novel bacteria-based self-healing concrete. Cem Concr Compos 33(7):763–770. https://doi.org/10.1016/j.cemconcomp.2011.03.012
Wu H, Yin J-J, Wamer WG, Zeng M, Lo YM (2014) Reactive oxygen species-related activities of nano-iron metal and nano-iron oxides. J Food Drug Anal 22(1):86–94. https://doi.org/10.1016/j.jfda.2014.01.007
Wu W, He Q, Jiang C (2008) Magnetic iron oxide nanoparticles: synthesis and surface functionalization strategies. Nanoscale Res Lett 3(11):397–415. https://doi.org/10.1007/s11671-008-9174-9
Wu W, Xiao X, Zhang S, Peng T, Zhou J, Ren F, Jiang C (2010) Synthesis and magnetic properties of maghemite (γ-Fe2O3) short-nanotubes. Nanoscale Res Lett 5(9):1474–1479. https://doi.org/10.1007/s11671-010-9664-4
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This investigation was financially supported by The University of Waikato, New Zealand.
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Seifan, M., Ebrahiminezhad, A., Ghasemi, Y. et al. Amine-modified magnetic iron oxide nanoparticle as a promising carrier for application in bio self-healing concrete. Appl Microbiol Biotechnol 102, 175–184 (2018). https://doi.org/10.1007/s00253-017-8611-z
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DOI: https://doi.org/10.1007/s00253-017-8611-z