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Geographic and environmental sources of variation in bacterial community composition in a large-scale municipal landfill site in China

  • Environmental biotechnology
  • Published:
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Abstract

Little is known regarding how bacterial communities assemble at landfill, as well as how the environment shapes the composition of bacterial community. In this study, up to 42 refuse samples from a large-scale landfill in China were physicochemically and phylogenetically investigated. 16S ribosomal RNA (rRNA) gene-based Illumina MiSeq sequencing (nine samples) revealed that representatives of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Bacteroidetes were dominant in the refuse samples, which was similar to a previous study on landfill leachate by using 454 pyrosequencing. Although 741 operational taxonomic units (OTUs) were detected across all nine samples, 6 of these OTUs were detected in all of the data sets, suggesting difference between bacterial community structures. Geographical differences between the samples, irrespective of depths, were revealed by a principal component analysis (PCA) based on the terminal restriction fragment length polymorphism (TRFLP) profiles of 42 refuse samples. Redundancy analysis (RDA) suggested that environmental heterogeneity (pH, landfilling ages, and depths) and the abundance of bacteria (represented by 16S rRNA gene copy numbers) were the main drivers shaping the bacterial community structure.

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Acknowledgments

We thank the Natural Science Foundation of China (Grant No. 51578528), the Natural Science Foundation of Chongqing (Grant Nos. cstc2014jcyjA20006 and cstc2014yykfC20002), and Dean Innovation Foundation of Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science (Y33Z080O10) for their financial support. We would like to thank the staff of JCG Refuse landfill for assistance in field sampling.

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Authors and Affiliations

  1. Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No. 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, Chongqing, 400714, China

    Liyan Song, Hongjie Liu & Jing Xu

  2. Department of Geology and Geophysics, University of Utah, Salt Lake City, UT, 84112, USA

    Shu Yang

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  1. Liyan Song
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  2. Shu Yang
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  3. Hongjie Liu
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  4. Jing Xu
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Correspondence to Liyan Song.

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This article does not contain any studies with human participants performed by any of the authors.

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The authors declare that they have no competing interests.

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Song, L., Yang, S., Liu, H. et al. Geographic and environmental sources of variation in bacterial community composition in a large-scale municipal landfill site in China. Appl Microbiol Biotechnol 101, 761–769 (2017). https://doi.org/10.1007/s00253-016-7917-6

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  • DOI: https://doi.org/10.1007/s00253-016-7917-6

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