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Baeyer-Villiger oxidations: biotechnological approach

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Abstract

Baeyer-Villiger monooxygenases (BVMOs) are a very well-known and intensively studied class of flavin-dependent enzymes. Their substrate promiscuity, high chemo-, regio-, and enantioselectivity are prerequisites for the use in synthetic chemistry and should pave the way for successful industrial processes. Nonetheless, only a very limited number of industrial relevant transformations are known, mainly due to the lack of BVMOs stability and cofactor dependency. In this review, we focus on novel BVMO-mediated transformations, BVMOs in cascade type reactions, potential industrial applications, and how limitations have been tackled by the community. Special attention will be put on whole-cell immobilization strategies. We emphasize to bridge recent developments in fundamental research to industrial applications.

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Acknowledgments

This work was supported by the Slovak Grant Agency for Science VEGA 2/0090/16 and by the Slovak Research and Development Agency under contract no. APVV-15-0227. This publication is the result of the project implementation: applied research in the field of industrial biocatalysis, ITMS code: 26240220079 supported by the Research & Development Operational Programme funded by the ERDF. Dr. Florian Rudroff and Prof. Marko D. Mihovilovic thank the FWF (grant no. I723-N17, P24483-B20), TU Wien (ABC-TOP Anschubfinanzierung), and the COST action systems biocatalysis WG2 for financial support.

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Authors and Affiliations

  1. Department of Glycobiotechnology, Institute of Chemistry – Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia

    Marek Bučko, Peter Gemeiner, Andrea Schenkmayerová & Tomáš Krajčovič

  2. Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9/163, A-1060, Vienna, Austria

    Florian Rudroff & Marko D. Mihovilovič

Authors
  1. Marek Bučko
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  2. Peter Gemeiner
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  3. Andrea Schenkmayerová
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  4. Tomáš Krajčovič
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  5. Florian Rudroff
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  6. Marko D. Mihovilovič
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Correspondence to Peter Gemeiner or Florian Rudroff.

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Bučko, M., Gemeiner, P., Schenkmayerová, A. et al. Baeyer-Villiger oxidations: biotechnological approach. Appl Microbiol Biotechnol 100, 6585–6599 (2016). https://doi.org/10.1007/s00253-016-7670-x

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  • DOI: https://doi.org/10.1007/s00253-016-7670-x

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