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Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers

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Abstract

Burkholderia is an incredibly diverse and versatile Gram-negative genus, within which over 80 species have been formally named and multiple other genotypic groups likely represent new species. Phylogenetic analysis based on the 16S rRNA gene sequence and core genome ribosomal multilocus sequence typing analysis indicates the presence of at least three major clades within the genus. Biotechnologically, Burkholderia are well-known for their bioremediation and biopesticidal properties. Within this review, we explore the ability of Burkholderia to synthesise a wide range of antimicrobial compounds ranging from historically characterised antifungals to recently described antibacterial antibiotics with activity against multiresistant clinical pathogens. The production of multiple Burkholderia antibiotics is controlled by quorum sensing and examples of quorum sensing pathways found across the genus are discussed. The capacity for antibiotic biosynthesis and secondary metabolism encoded within Burkholderia genomes is also evaluated. Overall, Burkholderia demonstrate significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites.

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Acknowledgments

MB acknowledges funding from the Cardiff University Synthetic Biology initiative. CP is indebted to the Special Research Council of Ghent University. TC acknowledges funding from the Fund for Scientific Research-Flanders and the Interuniversity Attraction Poles Programme initiated by the Belgian Science Policy Office. EM is a recipient of funding from the Biotechnology and Biological Sciences Research Council (Grant BB/L021692/1). PV and TC acknowledge funding from the Industrial Research Fund of Ghent University (Grant F2015/IOF-ConcepTT/142).

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  1. Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000, Ghent, Belgium

    Eliza Depoorter, Charlotte Peeters & Peter Vandamme

  2. Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium

    Eliza Depoorter & Tom Coenye

  3. Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Main Building, Museum Avenue, Cardiff, UK

    Matt J. Bull & Eshwar Mahenthiralingam

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  1. Eliza Depoorter
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Depoorter, E., Bull, M.J., Peeters, C. et al. Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers. Appl Microbiol Biotechnol 100, 5215–5229 (2016). https://doi.org/10.1007/s00253-016-7520-x

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