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Elicitors’ influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions

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Abstract

Cobalt nitrate, nickel sulphate, hydrogen peroxide, sodium nitroprusside, and culture filtrates of Pseudomonas monteili, Bacillus circularans, Trichoderma atroviridae, and Trichoderma harzianum were tested to elicit ginsenoside production in a cell suspension line of Panax quinquefolius. Abiotic elicitors preferentially increased panaxadiols whereas biotic elicitors upregulated the panaxatriol synthesis. Cobalt nitrate (50 μM) increased total ginsenosides content by twofold (54.3 mg/L) within 5 days. It also induced the Rc synthesis that was absent in the control cultures. Elicitation with P. monteili (2.5 % v/v, 5 days) also supported 2.4-fold enhancement in saponin yield. Elicitation by T. atroviridae or hydrogen peroxide induced the synthesis of Rg3 and Rh2 that are absent in ginseng roots. The highest ginsenosides productivity (3.2-fold of control) was noticed in cells exposed to 1.25 % v/v dose of T. atroviridae for 5 days. Treating cells with T. harzianum for 15 days afforded maximum synthesis and leaching (8.1 mg/L) of ginsenoside Rh1.

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Acknowledgments

The authors are grateful to the Director, CSIR-CIMAP, for providing the necessary infrastructure required for the present investigation. Tanya Biswas also acknowledges the University Grants Commission for the award of a Senior Research Fellowship.

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Authors and Affiliations

  1. Plant Biotechnology Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India

    Tanya Biswas, A. K. Mathur & Archana Mathur

  2. Microbiology and Entomology Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India

    Alok Kalra

  3. Genetics and Plant Breeding Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India

    R. K. Lal

  4. Analytical Chemistry Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial Research, PO CIMAP, Lucknow, 226015, India

    Manju Singh

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  1. Tanya Biswas
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Correspondence to Archana Mathur.

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Conflict of interest

Tanya Biswas declares that she has no conflict of interest. Alok Kalra declares that he has no conflict of interest. A K Mathur declares that he has no conflict of interest. R K Lal declares that he has no conflict of interest. Manju Singh declares that she has no conflict of interest. Archana Mathur declares that she has no conflict of interest.

Funding

This study was partially funded by a CSIR network project- BSC0117, entitled “Plant microbe and soil interactions.”

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Biswas, T., Kalra, A., Mathur, A.K. et al. Elicitors’ influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions. Appl Microbiol Biotechnol 100, 4909–4922 (2016). https://doi.org/10.1007/s00253-015-7264-z

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  • DOI: https://doi.org/10.1007/s00253-015-7264-z

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