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Dysbiosis of the fecal microbiota in the TNBS-induced Crohn’s disease mouse model

  • Applied genetics and molecular biotechnology
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Abstract

Crohn’s disease (CD) is characterized by chronic transmural inflammation. The symptom of the mice model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) is closed to human under CD condition, so this kind of animal is widely used in the related researches. Although the dysbiosis of the fecal microbiota has been proved to play an important role in the patients with CD, the composition of the gastrointestinal microbiota in the mouse model under disease condition is still unclear. In the current study, male 7-week BALB/c mice were anesthetized and intrarectal administrated by ethanol (ET group), TNBS in ethanol (TN group), and phosphate buffered saline (PBS) (CK group) as control. The symptoms of individuals under the CD condition were observed, and the changes of the bacterial taxonomic structure and functional composition were revealed by next-generation sequencing (NGS) 16S sequencing. The BALB/c mice in TN group demonstrated CD-like symptoms and the damages in the intestinal tract. The NGS 16S results exhibited that the diversity and microbial composition under CD condition are significantly different with those in ET group. The KEGG Orthology (KO) profile were generated from PICRUSt, and function modules such as methanogenesis (M00347) and microcin C transport system (M00349) were found enriched in the individuals in the TN group. This study proved that mouse model induced by TNBS could develop the similar symptom to CD patient, and we firstly showed the significant intestinal microbe changes on both taxonomic structure and functional composition in this mouse model.

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Acknowledgments

The study was supported by grants from National Nature Science Foundation of China (81470795), the Shenzhen Municipal Government of China (JSGG20140702161403250), the Shenzhen Engineering Laboratory of Detection and Intervention of human intestinal microbiome (DRC-SZ[2015]162), the Shenzhen Key Laboratory of Human commensal microorganisms and Health Research (CXB201108250098A), and the Shenzhen Municipal Government of China (the research and development of the novel personalized gut microbiota probiotic production, CXZZ20150330171521403).

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Authors and Affiliations

  1. Department of Gastroenterology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510655, China

    Qing He & Ying Li

  2. Nutrition Department, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510655, China

    Lingling Li & Ting Yan

  3. BGI-Shenzhen, Shenzhen, 518083, China

    Xiaoping Li, Chuan Liu, Lili Su, Zhongkui Xia, Xin Li, Qiang Feng & Liang Xiao

  4. Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research, Shenzhen, 518083, China

    Chuan Liu, Lili Su, Zhongkui Xia & Liang Xiao

  5. Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, Shenzhen, 518083, China

    Qiang Feng

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  1. Qing He
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  2. Xiaoping Li
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  3. Chuan Liu
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  4. Lili Su
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  5. Zhongkui Xia
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  6. Xin Li
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  7. Ying Li
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  8. Lingling Li
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  9. Ting Yan
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  10. Qiang Feng
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  11. Liang Xiao
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Correspondence to Qiang Feng or Liang Xiao.

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Animal experiments were performed in accordance with the guidelines of The Institutional Review Board on Bioethics and Biosafety of BGI (Number 13038).

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The authors declare that they have no conflicts of interests.

Additional information

Qing He, Xiaoping Li, Chuan Liu and Lili Su are contributed equally to this work.

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He, Q., Li, X., Liu, C. et al. Dysbiosis of the fecal microbiota in the TNBS-induced Crohn’s disease mouse model. Appl Microbiol Biotechnol 100, 4485–4494 (2016). https://doi.org/10.1007/s00253-015-7205-x

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