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Genotype-driven isolation of enterocin with novel bioactivities from mangrove-derived Streptomyces qinglanensis 172205

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Abstract

The type II polyketide synthase (PKS) natural product enterocin (1) was isolated from a mangrove-derived novel species Streptomyces qinglanensis 172205 guided by genome sequence, and its putative biosynthetic gene cluster was revealed. Its natural analogues 5-deoxyenterocin (2) and wailupemycin A–C (3–5) were also identified by tandem mass spectrometry. By feeding experiments with aryl acids, strain 172205 was proved to incorporate partial exogenous starter units into enterocin- and wailupemycin-based analogues, thus being a new and suitable microorganism for engineering unnatural enc-derived polyketide metabolites. In addition, biological assays indicated that enterocin showed obvious inhibitory activity against β-amyloid protein (Aβ1-42) fibrillation and moderate cytotoxicity against HeLa and HepG2 for the first time.

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Acknowledgments

This work was partially supported by the China “973” program (2012CB721001), “863” Program (2012AA092201), the National Natural Science Foundation of China (No. 31170467), and the EU FP7 project PharmaSea (312184).

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The authors confirm that this article content has no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China

    Dong-Bo Xu, Min Ma, Zi-Xin Deng & Kui Hong

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  1. Dong-Bo Xu
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  2. Min Ma
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  3. Zi-Xin Deng
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  4. Kui Hong
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Correspondence to Kui Hong.

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Xu, DB., Ma, M., Deng, ZX. et al. Genotype-driven isolation of enterocin with novel bioactivities from mangrove-derived Streptomyces qinglanensis 172205. Appl Microbiol Biotechnol 99, 5825–5832 (2015). https://doi.org/10.1007/s00253-015-6574-5

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  • DOI: https://doi.org/10.1007/s00253-015-6574-5

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