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Plasma membrane proteins Yro2 and Mrh1 are required for acetic acid tolerance in Saccharomyces cerevisiae

  • Applied microbial and cell physiology
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Abstract

Yro2 and its paralogous protein Mrh1 of Saccharomyces cerevisiae have seven predicted transmembrane domains and predominantly localize to the plasma membrane. Their physiological functions and regulation of gene expression have not yet been elucidated in detail. We herein demonstrated that MRH1 was constitutively expressed, whereas the expression of YRO2 was induced by acetic acid stress and entering the stationary phase. Fluorescence microscopic analysis revealed that Mrh1 and Yro2 were distributed as small foci in the plasma membrane under acetic acid stress conditions. The null mutants of these genes (mrh1∆, yro2∆, and mrh1∆yro2∆) showed delayed growth and a decrease in the productivity of ethanol in the presence of acetic acid, indicating that Yro2 and Mrh1 are involved in tolerance to acetic acid stress.

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Acknowledgments

We are grateful to Dr. D. Watanabe (Nara Inst. Sci. Technol.) for valuable discussion. This study was supported by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology (No. 26292039).

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Authors and Affiliations

  1. Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan

    Akiko Takabatake, Nozomi Kawazoe & Shingo Izawa

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  1. Akiko Takabatake
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  2. Nozomi Kawazoe
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  3. Shingo Izawa
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Correspondence to Shingo Izawa.

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Akiko Takabatake and Nozomi Kawazoe contributed equally to this work.

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Takabatake, A., Kawazoe, N. & Izawa, S. Plasma membrane proteins Yro2 and Mrh1 are required for acetic acid tolerance in Saccharomyces cerevisiae . Appl Microbiol Biotechnol 99, 2805–2814 (2015). https://doi.org/10.1007/s00253-014-6278-2

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