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A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities

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Abstract

Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology. We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies. To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed. The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l. Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium. The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity. Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date. SM-A87 EPS also showed high antioxidant activity. These results suggest that SM-A87 EPS has promising potentials in biotechnology.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grants 31290231, 31025001, 91228210, 31170055, 31270117, and 81271896), the Hi-Tech Research and Development Program of China (grants 2011AA090703, 2012AA092103, and 2012AA092105), and the China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (grants DY125-15-T-05 and DY125-15-R-03).

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, Shandong Province, China

    Mei-Ling Sun, Sheng-Bo Liu, Li-Ping Qiao, Xiu-Lan Chen, Xiuhua Pang, Mei Shi, Xi-Ying Zhang, Qi-Long Qin, Yu-Zhong Zhang & Bin-Bin Xie

  2. Marine Biotechnology Research Center, Shandong University, Jinan, 250100, Shandong Province, China

    Mei-Ling Sun, Sheng-Bo Liu, Li-Ping Qiao, Xiu-Lan Chen, Xiuhua Pang, Mei Shi, Xi-Ying Zhang, Qi-Long Qin, Bai-Cheng Zhou, Yu-Zhong Zhang & Bin-Bin Xie

  3. Collaborative Innovation Center of Deep Sea Biology, Shandong University, Jinan, 250100, Shandong Province, China

    Xiu-Lan Chen, Xiuhua Pang, Mei Shi, Xi-Ying Zhang, Qi-Long Qin, Yu-Zhong Zhang & Bin-Bin Xie

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  1. Mei-Ling Sun
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Correspondence to Bin-Bin Xie.

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Sun, ML., Liu, SB., Qiao, LP. et al. A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities. Appl Microbiol Biotechnol 98, 7437–7445 (2014). https://doi.org/10.1007/s00253-014-5839-8

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  • DOI: https://doi.org/10.1007/s00253-014-5839-8

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