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Laccase applications in biofuels production: current status and future prospects

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Abstract

The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisation is dependent on the efficient hydrolysis of polysaccharides, which in part is dependent on cost-effective and benign pretreatment of biomass to remove or modify lignin and release or expose sugars to hydrolytic enzymes. Laccase is one of the enzymes that are being investigated not only for potential use as pretreatment agents in biofuel production, mainly as a delignifying enzyme, but also as a biotechnological tool for removal of inhibitors (mainly phenolic) of subsequent enzymatic processes. The current review discusses the major advances in the application of laccase as a potential pretreatment strategy, the underlying principles as well as directions for future research in the search for better enzyme-based technologies for biofuel production. Future perspectives could include synergy between enzymes that may be required for optimal results and the adoption of the biorefinery concept in line with the move towards the global implementation of the bioeconomy strategy.

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Acknowledgments

Financial support from the National Research Foundation (NRF)—South Africa and the Cape Peninsula University of Technology Research Funding (URF) is gratefully acknowledged. Any opinion, findings and conclusions or recommendations expressed in this material are those of the author(s) and therefore the NRF does not accept any liability in regard thereto.

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  1. Biocatalysis and Technical Biology Research Group, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535, South Africa

    Tukayi Kudanga & Marilize Le Roes-Hill

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Correspondence to Tukayi Kudanga.

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Kudanga, T., Le Roes-Hill, M. Laccase applications in biofuels production: current status and future prospects. Appl Microbiol Biotechnol 98, 6525–6542 (2014). https://doi.org/10.1007/s00253-014-5810-8

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