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DNA barcoding to map the microbial communities: current advances and future directions

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Abstract

During the last two decades, the DNA barcode development towards microbial community has increased dramatically. DNA barcode development is related to error-free and quick species identification which aid in understanding the microbial biodiversity, as well as the diseases related to microbial species. Here, we seek to evaluate the so-called barcoding initiatives for the microbial communities and the emerging trends in this field. In this paper, we describe the development of DNA marker-based DNA barcoding system, comparison between routine species identification and DNA barcode, and microbial biodiversity and DNA barcode for microbial communities. Two major topics, such as the molecular diversity of viruses and barcode for viruses have been discussed at the same time. We demonstrate the current status and the maker of DNA barcode for bacteria, algae, fungi, and protozoa. Furthermore, we argue about the promises, limitations, and present and future challenges of microbial barcode development.

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Acknowledgments

The authors take this opportunity to thank the management of Galgotias University and VIT University for providing the facilities and encouragement to carry out this work. The authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Department of Bioinformatics, School of Computer and Information Sciences, Galgotias University, Greater Noida, India

    Chiranjib Chakraborty

  2. Medical Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India

    C. George Priya Doss

  3. Department of Zoology, Vidyasagar University, Midnapore, 721 102, West Bengal, India

    Bidhan C. Patra

  4. Machine Intelligence Unit, Indian Statistical Institute, Kolkata, India

    Sanghamitra Bandyopadhyay

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  1. Chiranjib Chakraborty
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  2. C. George Priya Doss
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  3. Bidhan C. Patra
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  4. Sanghamitra Bandyopadhyay
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Correspondence to Chiranjib Chakraborty.

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Chakraborty, C., Doss, C.G.P., Patra, B.C. et al. DNA barcoding to map the microbial communities: current advances and future directions. Appl Microbiol Biotechnol 98, 3425–3436 (2014). https://doi.org/10.1007/s00253-014-5550-9

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