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Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions

  • Applied microbial and cell physiology
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Abstract

Saccharomyces cerevisiae plays a primordial role in alcoholic fermentation and has a vast worldwide application in the production of fuel-ethanol, food and beverages. The dominance of S. cerevisiae over other microbial species during alcoholic fermentations has been traditionally ascribed to its higher ethanol tolerance. However, recent studies suggested that other phenomena, such as microbial interactions mediated by killer-like toxins, might play an important role. Here we show that S. cerevisiae secretes antimicrobial peptides (AMPs) during alcoholic fermentation that are active against a wide variety of wine-related yeasts (e.g. Dekkera bruxellensis) and bacteria (e.g. Oenococcus oeni). Mass spectrometry analyses revealed that these AMPs correspond to fragments of the S. cerevisiae glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein. The involvement of GAPDH-derived peptides in wine microbial interactions was further sustained by results obtained in mixed cultures performed with S. cerevisiae single mutants deleted in each of the GAPDH codifying genes (TDH1-3) and also with a S. cerevisiae mutant deleted in the YCA1 gene, which codifies the apoptosis-involved enzyme metacaspase. These findings are discussed in the context of wine microbial interactions, biopreservation potential and the role of GAPDH in the defence system of S. cerevisiae.

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Acknowledgments

The present work was financed by FEDER funds through POFC-COMPETE and by national funds through Fundação para a Ciência e a Tecnologia (FCT) in the scope of project FCOMP-01-0124-FEDER-014055. M.G.A. and J.C. acknowledge the funding support from FCT (PEst-C/EQB/LA0006/2011). Patrícia Branco is the recipient of a PhD fellowship (SFRH/BD/89673/2012) funded by FCT, Portugal. We want also to thanks to Professors Isabel Sá-Correia (IST/UTL, Lisbon, Portugal), Luísa Marinho (FCUL, Lisbon, Portugal) and Paula Ludovico (ICVS/UM, Braga, Portugal) for kindly providing some mutant strains.

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Authors and Affiliations

  1. Unit of Bioenergy, Laboratório Nacional de Energia e Geologia (LNEG), Estrada do Paço do Lumiar 22, 1649-038, Lisbon, Portugal

    Patrícia Branco, Diana Francisco & Helena Albergaria

  2. INRA, Plate-Forme d’Exploration du Métabolisme composante protéomique (PFEMcp), 63122, Saint-Genès Champanelle, France

    Christophe Chambon & Michel Hébraud

  3. Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, 1958, Frederiksberg C, Denmark

    Nils Arneborg

  4. REQUIMTE—Departamento de Química, Faculdade de Ciências e Tecnologia (UNL), 2829-516, Monte de Caparica, Portugal

    Maria Gabriela Almeida & Jorge Caldeira

  5. Instituto Superior de Saúde Egas Moniz, Campus Universitário, Quinta da Granja, 2829-511, Monte de Caparica, Portugal

    Maria Gabriela Almeida & Jorge Caldeira

Authors
  1. Patrícia Branco
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  2. Diana Francisco
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  3. Christophe Chambon
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  4. Michel Hébraud
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  5. Nils Arneborg
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  6. Maria Gabriela Almeida
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  7. Jorge Caldeira
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  8. Helena Albergaria
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Correspondence to Helena Albergaria.

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Branco, P., Francisco, D., Chambon, C. et al. Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions. Appl Microbiol Biotechnol 98, 843–853 (2014). https://doi.org/10.1007/s00253-013-5411-y

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  • DOI: https://doi.org/10.1007/s00253-013-5411-y

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