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Saccharomyces cerevisiae CCMI 885 secretes peptides that inhibit the growth of some non-Saccharomyces wine-related strains

  • Applied Microbial and Cell Physiology
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Abstract

The nature of the toxic compounds produced by Saccharomyces cerevisiae CCMI 885 that induce the early death of Hanseniaspora guilliermondii during mixed fermentations, as well as their ability to inhibit the growth of other non-Saccharomyces wine-related strains, was investigated. The killing effect of mixed supernatants towards H. guilliermondii was inactivated by protease treatments, thus revealing the proteinaceous nature of the toxic compounds. Analysis of the protein pattern of mixed supernatants on Tricine SDS-PAGE showed that this S. cerevisiae strain secretes peptides (<10 kDa), which were detected only when death of H. guilliermondii was already established. Death-inducing supernatants were ultrafiltrated by 10 and 2 kDa membranes, respectively, and the inhibitory effect of those permeates were tested in H. guilliermondii cultures. Results indicated that the (2–10) kDa protein fraction of those supernatants seemed to contain antimicrobial peptides active against H. guilliermondii. Thus, the (2–10) kDa protein fraction was concentrated and its inhibitory effect tested against strains of Kluyveromyces marxianus, Kluyveromyces thermotolerans, Torulaspora delbrueckii and H. guilliermondii. Under the growth conditions used for these tests, the (2–10) kDa protein fraction of S. cerevisiae CCMI 885 supernatants exhibited a fungistatic effect against all the strains and a fungicidal effect against K. marxianus.

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Acknowledgements

The present work was supported by project POCTI/AGR/39974/2001, funded by Fundação para a Ciência e Tecnologia.

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Authors and Affiliations

  1. Unidade de Bioenergia, LNEG, Estrada do Paço do Lumiar, 22, 1649-038, Lisboa, Portugal

    Helena Albergaria, Diana Francisco & Francisco Gírio

  2. Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, 1958, Frederiksberg C, Denmark

    Klaus Gori & Nils Arneborg

Authors
  1. Helena Albergaria
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  2. Diana Francisco
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  3. Klaus Gori
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  4. Nils Arneborg
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  5. Francisco Gírio
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Correspondence to Helena Albergaria.

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Albergaria, H., Francisco, D., Gori, K. et al. Saccharomyces cerevisiae CCMI 885 secretes peptides that inhibit the growth of some non-Saccharomyces wine-related strains. Appl Microbiol Biotechnol 86, 965–972 (2010). https://doi.org/10.1007/s00253-009-2409-6

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  • DOI: https://doi.org/10.1007/s00253-009-2409-6

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