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Recent advances in recombinant protein expression by Corynebacterium, Brevibacterium, and Streptomyces: from transcription and translation regulation to secretion pathway selection

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Abstract

Gram-positive bacteria are widely used to produce recombinant proteins, amino acids, organic acids, higher alcohols, and polymers. Many proteins have been expressed in Gram-positive hosts such as Corynebacterium, Brevibacterium, and Streptomyces. The favorable and advantageous characteristics (e.g., high secretion capacity and efficient production of metabolic products) of these species have increased the biotechnological applications of bacteria. However, owing to multiplicity from genes encoding the proteins and expression hosts, the expression of recombinant proteins is limited in Gram-positive bacteria. Because there is a very recent review about protein expression in Bacillus subtilis, here we summarize recent strategies for efficient expression of recombinant proteins in the other three typical Gram-positive bacteria (Corynebacterium, Brevibacterium, and Streptomyces) and discuss future prospects. We hope that this review will contribute to the development of recombinant protein expression in Corynebacterium, Brevibacterium, and Streptomyces.

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Long Liu, Haiquan Yang, Jianghua Li & Guocheng Du

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Long Liu, Haiquan Yang, Jianghua Li & Guocheng Du

  3. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, 30332, GA, USA

    Hyun-dong Shin

  4. National Engineering of Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Jian Chen

Authors
  1. Long Liu
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  2. Haiquan Yang
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  3. Hyun-dong Shin
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  4. Jianghua Li
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  5. Guocheng Du
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  6. Jian Chen
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Correspondence to Long Liu or Jian Chen.

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Liu, L., Yang, H., Shin, Hd. et al. Recent advances in recombinant protein expression by Corynebacterium, Brevibacterium, and Streptomyces: from transcription and translation regulation to secretion pathway selection. Appl Microbiol Biotechnol 97, 9597–9608 (2013). https://doi.org/10.1007/s00253-013-5250-x

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  • DOI: https://doi.org/10.1007/s00253-013-5250-x

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