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Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology

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Abstract

Increasing concerns over limited petroleum resources and associated environmental problems are motivating the development of efficient cell factories to produce chemicals, fuels, and materials from renewable resources in an environmentally sustainable economical manner. Bacillus spp., the best characterized Gram-positive bacteria, possesses unique advantages as a host for producing microbial enzymes and industrially important biochemicals. With appropriate modifications to heterologous protein expression and metabolic engineering, Bacillus species are favorable industrial candidates for efficiently converting renewable resources to microbial enzymes, fine chemicals, bulk chemicals, and fuels. Here, we summarize the recent advances in developing Bacillus spp. as a cell factory. We review the available genetic tools, engineering strategies, genome sequence, genome-scale structure models, proteome, and secretion pathways, and we list successful examples of enzymes and industrially important biochemicals produced by Bacillus spp. Furthermore, we highlight the limitations and challenges in developing Bacillus spp. as a robust and efficient production host, and we discuss in the context of systems and synthetic biology the emerging opportunities and future research prospects in developing Bacillus spp. as a microbial cell factory.

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Acknowledgments

This project was financially supported by the Enterprise-university-research prospective program, Jiangsu Province (BY2012054), 111 Project (111-2-06), 863 Program (2012AA022202), and 973 Program (2012CB720806, 2013CB733602).

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Long Liu, Yanfeng Liu, Jianghua Li & Guocheng Du

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Long Liu, Yanfeng Liu, Jianghua Li & Guocheng Du

  3. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, 30332, USA

    Hyun-dong Shin & Rachel R. Chen

  4. Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, 20742, USA

    Nam Sun Wang

  5. National Engineering of Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Jian Chen

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  1. Long Liu
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  2. Yanfeng Liu
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  3. Hyun-dong Shin
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  4. Rachel R. Chen
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  5. Nam Sun Wang
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  6. Jianghua Li
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  7. Guocheng Du
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  8. Jian Chen
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Correspondence to Guocheng Du or Jian Chen.

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Long Liu and Yanfeng Liu contributed equally.

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Liu, L., Liu, Y., Shin, Hd. et al. Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology. Appl Microbiol Biotechnol 97, 6113–6127 (2013). https://doi.org/10.1007/s00253-013-4960-4

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  • DOI: https://doi.org/10.1007/s00253-013-4960-4

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