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Identification of a novel enzymatic activity from lactic acid bacteria able to degrade biogenic amines in wine

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Abstract

The main objectives of this study were the search for enzymatic activities responsible for biogenic amine (BA) degradation in lactic acid bacteria (LAB) strains isolated from wine, their identification, and the evaluation of their applicability for reducing BAs in wine. Fifty-three percent of the 76 LAB cell extracts showed activity against a mixture of histamine, tyramine, and putrescine when analyzed in-gel. The quantification of the degrading ability for each individual amine was tested in a synthetic medium and wine. Most of the bacteria analyzed were able to degrade the three amines in both conditions. The highest percentages of degradation in wine were those of putrescine: up to 41 % diminution in 1 week. Enzymes responsible for amine degradation were isolated and purified from Lactobacillus plantarum J16 and Pediococcus acidilactici CECT 5930 strains and were identified as multicopper oxidases. This is the first report of an efficient BA reduction in wine by LAB. Furthermore, the identity of the enzymes involved has been revealed.

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Acknowledgments

The authors gratefully acknowledge support from this work from the Ministerio de Educación y Ciencia, Spain (projects AGL2006-08495 and AGL2009-12167), ERDF funds, and the City Hall of Valencia. We also thank Dr. Graciela Vignolo for providing us with Lactobacillus farciminis CRL 678 (provided as L. casei), and Artur Roig-Sagués for providing us with Lactobacillus curvatus C9-19C and C13-48. This research has been performed within the Programme VLC/Campus, Microcluster IViSoCa (Innovation for a Sustainable and Quality Viticulture). ENOLAB participates in the ERI BioTechMed from the Universitat de València. English text was revised by Beverly Johnson.

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  1. ENOLAB, Departament de Microbiologia i Ecologia, Universitat de València, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain

    S. Callejón, S. Ferrer & I. Pardo

  2. Departament de Bioquímica i Biologia Molecular, Universitat de València, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain

    R. Sendra

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  1. S. Callejón
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  4. I. Pardo
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Correspondence to I. Pardo.

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Callejón, S., Sendra, R., Ferrer, S. et al. Identification of a novel enzymatic activity from lactic acid bacteria able to degrade biogenic amines in wine. Appl Microbiol Biotechnol 98, 185–198 (2014). https://doi.org/10.1007/s00253-013-4829-6

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  • DOI: https://doi.org/10.1007/s00253-013-4829-6

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