Abstract
Microorganism in drinking water distribution system may colonize in biofilms. Bacterial 16S rRNA gene diversities were analyzed in both water and biofilms grown on taps with three different materials (polyvinyl chloride (PVC), stainless steel, and cast iron) from a local drinking water distribution system. In total, five clone libraries (440 sequences) were obtained. The taxonomic composition of the microbial communities was found to be dominated by members of Proteobacteria (65.9–98.9 %), broadly distributed among the classes Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. Other bacterial groups included Firmicutes, Acidobacteria, Bacteroidetes, Cyanobacteria, and Deinococcus-Thermus. Moreover, a small proportion of unclassified bacteria (3.5–10.6 %) were also found. This investigation revealed that the bacterial communities in biofilms appeared much more diversified than expected and more care should be taken to the taps with high bacterial diversity. Also, regular monitor of outflow water would be useful as potentially pathogenic bacteria were detected. In addition, microbial richness and diversity in taps ranked in the order as: PVC < stainless steel < cast iron. All the results interpreted that PVC would be a potentially suitable material for use as tap component in drinking water distribution system.
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Acknowledgments
We express gratitude to Ms. Ping Wang, Mr. Zhimin Yu, and members of the local drinking water plant for helping in sampling. This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (Y025014EA2) and the Natural Science Foundation of China (No.51208501).
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Lin, W., Yu, Z., Chen, X. et al. Molecular characterization of natural biofilms from household taps with different materials: PVC, stainless steel, and cast iron in drinking water distribution system. Appl Microbiol Biotechnol 97, 8393–8401 (2013). https://doi.org/10.1007/s00253-012-4557-3
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DOI: https://doi.org/10.1007/s00253-012-4557-3