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Substrate oxidation by dye-decolorizing peroxidases (DyPs) from wood- and litter-degrading agaricomycetes compared to other fungal and plant heme-peroxidases

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Abstract

Catalytic and physicochemical properties of representative fungal dye-decolorizing peroxidases (DyPs) of wood- (WRF) and litter-decomposing white-rot fungi (LDF) are summarized and compared, including one recombinant Mycetinis scorodonius DyP (rMscDyP; LDF), the wild-type Auricularia auricula-judae DyP (AauDyP; WRF), and two new DyPs secreted by the jelly fungi Exidia glandulosa (EglDyP; WRF) and Mycena epipterygia (MepDyP; LDF). Homogeneous preparations of these DyPs were obtained after different steps of fast protein liquid chromatography, and they increase the total number of characterized fungal DyP proteins to eight. The peptide sequences of AauDyP, MepDyP, and EglDyP showed highest homologies (52–56 %) to the DyPs of M. scorodonius. Five out of the eight characterized fungal DyPs were used to evaluate their catalytic properties compared to classic fungal and plant heme peroxidases, namely lignin peroxidase of Phanerochaete chrysosporium (PchLiP; WRF), versatile peroxidase of Bjerkandera adusta (BadVP; WRF), and generic peroxidases of Coprinopsis cinerea (CiP) and Glycine max (soybean peroxidase = SBP). All DyPs tested possess unique properties regarding the stability at low pH values: 50–90 % enzymatic activity remained after 4-h exposition at pH 2.5, and the oxidation of nonphenolic aromatic substrates (lignin model compounds) was optimal below pH 3. Furthermore, all DyPs efficiently oxidized recalcitrant dyes (e.g., Azure B) as well as the phenolic substrate 2,6-dimethoxyphenol. Thus, DyPs combine features of different peroxidases on the functional level and may be part of the biocatalytic system secreted by fungi for the oxidation of lignin and/or toxic aromatic compounds.

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Acknowledgment

The work has been partly funded by the European Union (integrated projects Biorenew and Peroxicats), the Deutsche Bundesstiftung Umwelt (DBU, project 13211-032 “Pilzliche Sekretome”), the DFG Priority Program 1374 “Infrastructure-Biodiversity-Exploratories” (HO 1961/4–1) (Deutsche Forschungsgemeinschaft, projects Fupers and Funwood), the Deutscher Akademischer Austauschdienst (DAAD, PPP 50151083), and the Bundesministerium für Bildung und Forschung (BMBF, VNM 09/014). We thank K. Piontek, D. Plattner, and E. Strittmatter for useful comments and their know-how in peroxidase crystal structures as well as our coworkers I. Kluge, M. Kinne, M. Poraj-Kobielska, S. Peter, C. Dolge, T. Arnstadt, D.H. Nghi, and K. Barková for their help in the lab and useful discussions.

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  1. Department of Bio- and Environmental Sciences, International Graduate School of Zittau, Markt 23, 02763, Zittau, Germany

    Christiane Liers, Marek J. Pecyna, Harald Kellner, Anja Worrich, Martin Hofrichter & René Ullrich

  2. Justus Liebig University Giessen, Heinrich-Buff-Ring 58, 35392, Giessen, Germany

    Holger Zorn

  3. Department of Food and Environmental Sciences, Viiki Biocenter, University of Helsinki, Viikinkaari 9, 00014, Helsinki, Finland

    Kari T. Steffen

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  1. Christiane Liers
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Liers, C., Pecyna, M.J., Kellner, H. et al. Substrate oxidation by dye-decolorizing peroxidases (DyPs) from wood- and litter-degrading agaricomycetes compared to other fungal and plant heme-peroxidases. Appl Microbiol Biotechnol 97, 5839–5849 (2013). https://doi.org/10.1007/s00253-012-4521-2

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