Abstract
Antrodia camphorata is a well-known Chinese medicinal mushroom that protects against diverse health-related conditions. Submerged fermentation of A. camphorata is an alternative choice for the effective production of bioactive metabolites, but the effects of nutrition and environment on mycelial morphology are largely unknown. In this study, we show that A. camphorata American Type Culture Collection 200183 can form arthrospores in the end of liquid fermentation. Different morphologies of A. camphorata in submerged culture were analyzed using scanning electron microscopy. The optimal carbon and nitrogen sources for sporulation were soluble starch and yeast extract. We found that a carbon-to-nitrogen ratio (C/N) of 40:1, MgSO4 (0.5 g/l), KH2PO4 (3.0 g/l), an initial pH 5.0, and an inoculum size of 1.5 × 105 spores/ml led to maximum production of arthroconidia. Our results will be useful in the regulation and optimization of A. camphorata cultures for efficient production of arthroconidia in submerged culture, which can be used as inocula in subsequent fermentation processes.
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Acknowledgments
This work was supported by the grants from the Natural Science Foundation of Jiangsu Province, China (grant no. BK2010142), National High-Tech Program of China (grant no. 2007AA021506), Fundamental Research Funds for the Central Universities (grant nos. JUSRP211A42 and JUSRP211A43), Industry-Academia Cooperation Innovation Fund Project of Jiangsu Province, China (grant no. BY2012052), and National Natural Science Foundation of China (grant no. 31201020).
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Yan Geng and Zhe He contributed equally to this work.
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Geng, Y., He, Z., Lu, ZM. et al. Antrodia camphorata ATCC 200183 sporulates asexually in submerged culture. Appl Microbiol Biotechnol 97, 2851–2858 (2013). https://doi.org/10.1007/s00253-012-4513-2
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DOI: https://doi.org/10.1007/s00253-012-4513-2