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Extractive biodecolorization of triphenylmethane dyes in cloud point system by Aeromonas hydrophila DN322p

  • Environmental biotechnology
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Abstract

The biological treatment of triphenylmethane dyes is an important issue. Most microbes have limited practical application because they cannot completely detoxicate these dyes. In this study, the extractive biodecolorization of triphenylmethane dyes by Aeromonas hydrophila DN322p was carried out by introducing the cloud point system. The cloud point system is composed of a mixture of nonionic surfactants (20 g/L) Brij 30 and Tergitol TMN-3 in equal proportions. After the decolorization of crystal violet, a higher wet cell weight was obtained in the cloud point system than that of the control system. Based on the results of thin-layer chromatography, the residual crystal violet and its decolorized product, leuco crystal violet, preferred to partition into the coacervate phase. Therefore, the detoxification of the dilute phase was achieved, which indicated that the dilute phase could be discharged without causing dye pollution. The extractive biodecolorization of three other triphenylmethane dyes was also examined in this system. The decolorization of malachite green and brilliant green was similar to that of crystal violet. Only ethyl violet achieved a poor decolorization rate because DN322p decolorized it via adsorption but did not convert it into its leuco form. This study provides potential application of biological treatment in triphenylmethane dye wastewater.

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Acknowledgments

We acknowledge the National Basic Research Program of China (973 Program) (2012CB22307), the Team Project of the Natural Science Foundation of Guangdong Province (9351007002000001), the Natural Science Foundation of Guangdong Province (S2011010004267; 9251007002000003), the Outstanding Scholarship Foundation of Guangdong Academy of Sciences (200902), the Guangdong–Hongkong Technology Cooperation Funding (2009A030902003), Guangdong Province–Chinese Academy of Sciences strategic cooperative project (2009B091300023, 2010B090301048), and the International Cooperation Projects of Guangdong Province (2011B050400005).

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  1. Meiying Xu

    Present address: Guangdong Institute of Microbiology, Guangzhou, 510070, China

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, 510070, People’s Republic of China

    Tao Pan, Suizhou Ren, Meiying Xu, Guoping Sun & Jun Guo

  2. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People’s Republic of China

    Tao Pan

  3. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, 510070, People’s Republic of China

    Tao Pan, Suizhou Ren, Meiying Xu, Guoping Sun & Jun Guo

  4. State Key Laboratory of Applied Microbiology (Ministry—Guangdong Province Jointly Breeding Base), Guangzhou, South China, 510070, People’s Republic of China

    Tao Pan, Suizhou Ren, Meiying Xu, Guoping Sun & Jun Guo

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  1. Tao Pan
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  2. Suizhou Ren
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Correspondence to Meiying Xu.

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Pan, T., Ren, S., Xu, M. et al. Extractive biodecolorization of triphenylmethane dyes in cloud point system by Aeromonas hydrophila DN322p. Appl Microbiol Biotechnol 97, 6051–6055 (2013). https://doi.org/10.1007/s00253-012-4422-4

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  • DOI: https://doi.org/10.1007/s00253-012-4422-4

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