Abstract
Extractive microbial transformation of L-phenylacetylcarbinol (L-PAC) in nonionic surfactant Triton X-100 micelle aqueous solution was investigated by response surface methodology. Based on the Box–Behnken design, a mathematical model was developed for the predication of mutual interactions between benzaldehyde, Triton X-100, and glucose on L-PAC production. It indicated that the negative or positive effect of nonionic surfactant strongly depended on the substrate concentration. The model predicted that the optimal concentration of benzaldehyde, Triton X-100, and glucose was 1.2 ml, 15 g, and 2.76 g per 100 ml, respectively. Under the optimal condition, the maximum L-PAC production was 27.6 mM, which was verified by a time course of extractive microbial transformation. A discrete fed-batch process for verification of cell activity was also presented.
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Acknowledgements
The project was financially supported by the National Natural Science Foundation of China (No. 20676080) and partially supported by the Open Project Program of the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China, and Morning Star Promotive Program for Young Scholar of Shanghai Jiao Tong University, Shanghai, China.
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Xue, Y., Qian, C., Wang, Z. et al. Investigation of extractive microbial transformation in nonionic surfactant micelle aqueous solution using response surface methodology. Appl Microbiol Biotechnol 85, 517–524 (2010). https://doi.org/10.1007/s00253-009-2139-9
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DOI: https://doi.org/10.1007/s00253-009-2139-9