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An efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain obtained through adaptive evolution and its global transcription profile

  • Bioenergy and biofuels
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Abstract

Factors related to ethanol production from xylose in engineered Saccharomyces cerevisiae that contain an exogenous initial metabolic pathway are still to be elucidated. In the present study, a strain that expresses the xylose isomerase gene of Piromyces sp. Pi-xylA and overexpresses XKS1, RPE1, RKI1, TAL1, and TKL1, with deleted GRE3 and COX4 genes was constructed. The xylose utilization capacity of the respiratory deficiency strain was poor but improved via adaptive evolution in xylose. The μ max of the evolved strain in 20 g l−1 xylose is 0.11 ± 0.00 h−1, and the evolved strain consumed 17.83 g l−1 xylose within 72 h, with an ethanol yield of 0.43 g g−1 total consumed sugars during glucose–xylose cofermentation. Global transcriptional changes and effect of several specific genes were studied. The result revealed that the increased xylose isomerase acivity, the upregulation of enzymes involved in glycolysis and glutamate synthesis, and the downregulation of trehalose and glycogen synthesis, may have contributed to the improved xylose utilization of the strain. Furthermore, the deletion of PHO13 decreased the xylose growth in the respiration deficiency strain although deleting PHO13 can improve the xylose metabolism in other strains.

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Acknowledgments

This work was supported by the National Key Basic Research Program (2011CB707405), the International S&T Cooperation Program of China (2010DFA32560), and the National Natural Science Foundation of China (30970091 and 31070096).We would like to thank Dr. Peter Kötter of the Johann Wolfgang Goethe-University in Frankfurt for supplying the CEN.PK strains.

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Authors and Affiliations

  1. The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road 27#, Jinan, 250100, China

    Yu Shen, Xiao Chen, Bingyin Peng, Liyuan Chen, Jin Hou & Xiaoming Bao

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  1. Yu Shen
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  2. Xiao Chen
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  3. Bingyin Peng
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  4. Liyuan Chen
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  5. Jin Hou
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  6. Xiaoming Bao
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Correspondence to Xiaoming Bao.

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Yu Shen and Xiao Chen are co-first authors.

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Shen, Y., Chen, X., Peng, B. et al. An efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain obtained through adaptive evolution and its global transcription profile. Appl Microbiol Biotechnol 96, 1079–1091 (2012). https://doi.org/10.1007/s00253-012-4418-0

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