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Characterization of a novel lipolytic enzyme from Aspergillus oryzae

  • Biotechnologically relevant enzymes and proteins
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Abstract

In this study, we report the characterization of a protein from Aspergillus oryzae, exhibiting sequence identity with paraben esterase from the genus Aspergillus. The coding region of 1,586 bp, including a 77-bp intron, encoded a protein of 502 amino acids. The gene without the signal peptide of 19 amino acids was cloned into a vector, pPICZαC, and expressed successfully in Pichia pastoris as an active extracellular protein. The purified recombinant protein had pH and temperature optima of 7.0–8.0 and 30 °C, respectively, and was stable at the pH range of 7.0–10.0 and up to 40 °C. The optimal substrate for hydrolysis by the purified recombinant protein, among a panel of α-naphthyl esters (C2–C16), was α-naphthyl butyrate (C4), with activity of 0.16 units/mg protein. The considerable hydrolytic activity of the purified recombinant enzyme toward tributyrin was determined. However, no paraben esterase activity was detected toward the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid. In addition, no activity was detected toward the methyl esters of ferulic, p-coumaric, caffeic, and sinapic acids that would indicate feruloyl esterase activity.

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Authors and Affiliations

  1. Faculty of Agriculture, Yamagata University, 1-23 Wakaba-machi, Tsuruoka, Yamagata, 997-8555, Japan

    Takuya Koseki, Shungo Asai & Yoshihito Shiono

  2. Advanced Biosciences, Keio University, 403-1 Nipponkoku, Daihoji, Tsuruoka, Yamagata, 997-0017, Japan

    Natsumi Saito, Masayo Mori, Yasuko Sakaguchi & Kazutaka Ikeda

  3. Department of Food and Applied Life Sciences, Faculty of Agriculture, Yamagata University, 1-23 Wakaba-machi, Tsuruoka, Yamagata, 997-8555, Japan

    Takuya Koseki

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  1. Takuya Koseki
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  2. Shungo Asai
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  3. Natsumi Saito
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  4. Masayo Mori
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  5. Yasuko Sakaguchi
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  6. Kazutaka Ikeda
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  7. Yoshihito Shiono
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Correspondence to Takuya Koseki.

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Koseki, T., Asai, S., Saito, N. et al. Characterization of a novel lipolytic enzyme from Aspergillus oryzae . Appl Microbiol Biotechnol 97, 5351–5357 (2013). https://doi.org/10.1007/s00253-012-4391-7

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  • DOI: https://doi.org/10.1007/s00253-012-4391-7

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