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Aspergillus and Penicillium identification using DNA sequences: barcode or MLST?

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Abstract

Current methods in DNA technology can detect single nucleotide polymorphisms with measurable accuracy using several different approaches appropriate for different uses. If there are even single nucleotide differences that are invariant markers of the species, we can accomplish identification through rapid DNA-based tests. The question of whether we can reliably detect and identify species of Aspergillus and Penicillium turns mainly upon the completeness of our alpha taxonomy, our species concepts, and how well the available DNA data coincide with the taxonomic diversity in the family Trichocomaceae. No single gene is yet known that is invariant within species and variable between species as would be optimal for the barcode approach. Data are published that would make an MLST approach to isolate identification possible in the most well-studied clades of Aspergillus and Penicillium.

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Acknowledgments

The author gratefully acknowledges the skilled technical support provided by Amy E. McGovern. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable. USDA is an equal opportunity provider and employer.

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  1. Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA

    Stephen W. Peterson

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Correspondence to Stephen W. Peterson.

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Peterson, S.W. Aspergillus and Penicillium identification using DNA sequences: barcode or MLST?. Appl Microbiol Biotechnol 95, 339–344 (2012). https://doi.org/10.1007/s00253-012-4165-2

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  • DOI: https://doi.org/10.1007/s00253-012-4165-2

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