Abstract
The selection of suitable reference genes is crucial for accurate quantification of gene expression. To identify suitable reference genes in Beauveria bassiana, the expression of 14 candidates (18S, 28S, β-Tub, GAPD, γ-Act, TEF, HGPT, His3, His2A, TBP, CypA, CypB, PP1, and CrzA) was measured by quantitative polymerase chain reaction at different development stages and under various nutritional and stress conditions. Expression stability, as evaluated by the geNorm and NormFinder programs, revealed that His2A/γ-Act/CrzA was the most stably expressed set of genes throughout development, while 28S/PP1/CypA and His2A/γ-Act/CypA were the most stably expressed gene sets under a variety of nutritional and stress conditions, respectively. Overall, the most stably expressed genes under all conditions examined were PP1, γ-Act, and CypA.
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Acknowledgments
This work was supported by grants from the Ministry of Science and Technology of China (2009CB118904). The authors would like to thank, Guang-Mao Shen for geNorm and NormFinder assistance, and Jin-Cheng Ma, Xiu-Ping Zou, and Xiao-Dong Jiang for critical reading of the manuscript.
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Zhou, YH., Zhang, YJ., Luo, ZB. et al. Selection of optimal reference genes for expression analysis in the entomopathogenic fungus Beauveria bassiana during development, under changing nutrient conditions, and after exposure to abiotic stresses. Appl Microbiol Biotechnol 93, 679–685 (2012). https://doi.org/10.1007/s00253-011-3561-3
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DOI: https://doi.org/10.1007/s00253-011-3561-3