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Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum

  • Applied genetics and molecular biotechnology
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Abstract

Corynebacterium glutamicum wild type lacks the ability to utilize the pentose fractions of lignocellulosic hydrolysates, but it is known that recombinants expressing the araBAD operon and/or the xylA gene from Escherichia coli are able to grow with the pentoses xylose and arabinose as sole carbon sources. Recombinant pentose-utilizing strains derived from C. glutamicum wild type or from the l-lysine-producing C. glutamicum strain DM1729 utilized arabinose and/or xylose when these were added as pure chemicals to glucose-based minimal medium or when they were present in acid hydrolysates of rice straw or wheat bran. The recombinants grew to higher biomass concentrations and produced more l-glutamate and l-lysine, respectively, than the empty vector control strains, which utilized the glucose fraction. Typically, arabinose and xylose were co-utilized by the recombinant strains along with glucose either when acid rice straw and wheat bran hydrolysates were used or when blends of pure arabinose, xylose, and glucose were used. With acid hydrolysates growth, amino acid production and sugar consumption were delayed and slower as compared to media with blends of pure arabinose, xylose, and glucose. The ethambutol-triggered production of up to 93 ± 4 mM l-glutamate by the wild type-derived pentose-utilizing recombinant and the production of up to 42 ± 2 mM l-lysine by the recombinant pentose-utilizing lysine producer on media containing acid rice straw or wheat bran hydrolysate as carbon and energy source revealed that acid hydrolysates of agricultural waste materials may provide an alternative feedstock for large-scale amino acid production.

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Acknowledgements

Work in the laboratories of the corresponding authors was supported in part by grants from the Department of Biotechnology (DBT), New Delhi, India, and from the International Bureau (IB) of the Federal Ministry of Education and Research (BMBF), Germany (IND 07/030) under the Indo-German bilateral program.

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Authors and Affiliations

  1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, 695 019, Kerala, India

    Vipin Gopinath & K. Madhavan Nampoothiri

  2. Chair of Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Universitaetsstr. 25, 33615, Bielefeld, Germany

    Tobias M. Meiswinkel & Volker F. Wendisch

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  1. Vipin Gopinath
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  2. Tobias M. Meiswinkel
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  3. Volker F. Wendisch
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  4. K. Madhavan Nampoothiri
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Correspondence to Volker F. Wendisch or K. Madhavan Nampoothiri.

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V. Gopinath and T. M. Meiswinkel contributed equally to this work.

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Gopinath, V., Meiswinkel, T.M., Wendisch, V.F. et al. Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum . Appl Microbiol Biotechnol 92, 985–996 (2011). https://doi.org/10.1007/s00253-011-3478-x

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