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Characterization of two β-xylosidases from Bifidobacterium adolescentis and their contribution to the hydrolysis of prebiotic xylooligosaccharides

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Abstract

Xylooligosaccharides have strong bifidogenic properties and are increasingly used as a prebiotic. Nonetheless, little is known about the degradation of these substrates by bifidobacteria. We characterized two recombinant β-xylosidases, XylB and XylC, with different substrate specificities from Bifidobacterium adolescentis. XylB is a novel β-xylosidase that belongs to the recently introduced glycoside hydrolase family 120. In contrast to most reported β-xylosidases, it shows only weak activity on xylobiose and prefers xylooligosaccharides with a degree of polymerization above two. The remaining xylobiose is efficiently hydrolyzed by the second B. adolescentis β-xylosidase, XylC, a glycoside hydrolase of family 43. Furthermore, XylB releases more xylose from arabinose-substituted xylooligosaccharides than XylC (30% and 20%, respectively). The different specificities of XylB, XylC, and the recently described reducing-end xylose-releasing exo-oligoxylanase RexA show how B. adolescentis can efficiently degrade prebiotic xylooligosaccharides.

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Acknowledgments

We thank C. Grootaert (Laboratory for Microbial Ecology and Technology, Ghent University, Belgium) for providing a genomic DNA sample of B. adolescentis. We gratefully acknowledge the financial support from the “Instituut voor de aanmoediging van Innovatie door Wetenschap en Technologie in Vlaanderen” (I.W.T., SBO IMPAXOS project funding) and the Research Fund K.U. Leuven (project IDO/03/005).

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  1. Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Box 2462, 3001, Leuven, Belgium

    Stijn Lagaert, Rob Lavigne & Guido Volckaert

  2. Laboratory of Food Chemistry and Biochemistry and Leuven Food Science and Nutrition Research Centre (LFoRCe), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Box 2463, 3001, Leuven, Belgium

    Annick Pollet, Jan A. Delcour & Christophe M. Courtin

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  1. Stijn Lagaert
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Correspondence to Christophe M. Courtin.

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Lagaert, S., Pollet, A., Delcour, J.A. et al. Characterization of two β-xylosidases from Bifidobacterium adolescentis and their contribution to the hydrolysis of prebiotic xylooligosaccharides. Appl Microbiol Biotechnol 92, 1179–1185 (2011). https://doi.org/10.1007/s00253-011-3396-y

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