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Effects of electron donors and acceptors on anaerobic reduction of azo dyes by Shewanella decolorationis S12

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Abstract

Shewanella decolorationis S12 was able to reduce various azo dyes in a defined medium with formate, lactate, and pyruvate or H2 as electron donors under anaerobic conditions. Purified membranous, periplasmic, and cytoplasmic fractions from strain S12 analyzed, respectively, only membranous fraction was capable of reducing azo dye in the presence of electron donor, indicating that the enzyme system for anaerobic azoreduction was located on cellular membrane. Respiratory inhibitor Cu2+, dicumarol, stigmatellin, and metyrapone inhibited anaerobic azoreduction by purified membrane fraction, suggesting that the bacterial anaerobic azoreduction by strain S12 was a biochemical process that oxidizes the electron donors and transfers the electrons to the acceptors through a multicompound system related to electron transport chain. Dehydrogenases, cytochromes, and menaquinones were essential electron transport components for the azoreduction. The electron transport process for azoreduction was almost fully inhibited by O2, 6 mM of \({\text{NO}}^{ - }_{3} \), and 0.9 mM of \({\text{NO}}^{ - }_{2} \), but not by 10 mM of Fe3+. The inhibition may be a result from the competition for electrons from electron donors. These findings impact on the understanding of the mechanism of bacterial anaerobic azoreduction and have implication for improving treatment methods of wastewater contaminated by azo dyes.

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Acknowledgements

This research was supported by Chinese National Programs for High Technology Research and Development (2003AA214040), Guangdong Provincial Programs for Natural Science Foundation Group (015017), and Guangdong Provincial key Programs for Science and Technology Development (05100365).

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Authors and Affiliations

  1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Yiguo Hong

  2. Guangdong Institute of Microbiology, Guangzhou, 510070, China

    Yiguo Hong, Xingjuan Chen, Jun Guo, Zhicheng Xu, Meiying Xu & Guoping Sun

  3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, 510070, China

    Yiguo Hong & Xingjuan Chen

  4. China Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Yiguo Hong & Xingjuan Chen

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  1. Yiguo Hong
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  2. Xingjuan Chen
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  3. Jun Guo
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  4. Zhicheng Xu
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Correspondence to Guoping Sun.

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Hong, Y., Chen, X., Guo, J. et al. Effects of electron donors and acceptors on anaerobic reduction of azo dyes by Shewanella decolorationis S12. Appl Microbiol Biotechnol 74, 230–238 (2007). https://doi.org/10.1007/s00253-006-0657-2

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  • DOI: https://doi.org/10.1007/s00253-006-0657-2

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