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Characterization of a bifunctional xylanase/endoglucanase from yak rumen microorganisms

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Abstract

A new gene, RuCelA, encoding a bifunctional xylanase/endoglucanase, was cloned from a metagenomic library of yak rumen microorganisms. RuCelA showed activity against xylan and carboxymethylcellulose (CMC), suggesting bifunctional xylanase/endoglucanase activity. The optimal conditions for xylanase and endoglucanase activities were 65°C, pH 7.0 and 50°C, pH 5.0, respectively. In addition, the presence of Co+ and Co2+ can greatly improve RuCelA's endoglucanase activity, while inhibits its xylanase activity. Further examination of substrate preference showed a higher activity against barley glucan and lichenin than against xylan and CMC. Using xylan and barley glucan as substrates, RuCelA displayed obvious synergistic effects with β-1,4-xylosidase and β-1,4-glucosidase. Generation of soluble oligosaccharides from lignocellulose is the key step in bioethanol production, and it is greatly notable that RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefore, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial applications.

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Acknowledgments

This work was supported by Chinese High-tech Research and Development Program 2007AA021302.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, 200433, People’s Republic of China

    Lei Chang, Lei Bao, Yingzhi Chen, Jungang Zhou & Hong Lu

  2. School of Pharmacy, China Pharmaceutical University, Nanjing, 211198, People’s Republic of China

    Mozhu Ding

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  1. Lei Chang
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  2. Mozhu Ding
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  3. Lei Bao
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  4. Yingzhi Chen
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  5. Jungang Zhou
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Correspondence to Jungang Zhou or Hong Lu.

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Chang, L., Ding, M., Bao, L. et al. Characterization of a bifunctional xylanase/endoglucanase from yak rumen microorganisms. Appl Microbiol Biotechnol 90, 1933–1942 (2011). https://doi.org/10.1007/s00253-011-3182-x

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  • DOI: https://doi.org/10.1007/s00253-011-3182-x

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