Abstract
We demonstrate glutamate production from β-glucan using endoglucanase (EG)-expressing Corynebacterium glutamicum. The signal sequence torA derived from Escherichia coli K12, which belongs to the Tat pathway, was suitable for secreting EG of Clostridium thermocellum using C. glutamicum as a host. Using the torA signal sequence, endoglucanase from Clostridium cellulovorans 743B was successfully expressed, and the secreted EG produced 123 mg of reducing sugar from 5 g of β-glucan at 30 °C for 72 h, which is the optimal condition for C. glutamicum growth. Subsequently, glutamate fermentation from β-glucan was carried out with the addition of Aspergillus aculeatus β-glucosidase produced by recombinant Aspergillus oryzae. Using EG-secreting C. glutamicum, 178 mg/l of glutamate was produced from 15 g of β-glucan. This is the first report of glutamate fermentation from β-glucan using endoglucanase-secreting C. glutamicum.
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Acknowledgments
This work was partially supported by a Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This work was also partially supported by a Grant-in-Aid for JSPS Fellows (20859 to T. Tateno).
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Tsuchidate, T., Tateno, T., Okai, N. et al. Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum . Appl Microbiol Biotechnol 90, 895–901 (2011). https://doi.org/10.1007/s00253-011-3116-7
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DOI: https://doi.org/10.1007/s00253-011-3116-7