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Extending the alkene substrate range of vinyl chloride utilizing Nocardioides sp. strain JS614 with ethene oxide

  • Applied Microbial and Cell Physiology
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Abstract

Nocardioides sp. strain JS614 grows on the C2 alkenes ethene (Eth), vinyl chloride, and vinyl fluoride as sole carbon sources. The presence of 400–800 μM ethene oxide (EtO) extended the growth substrate range to propene (C3) and butene (C4). Propene-dependent growth of JS614 was CO2 dependent and was prevented by the carboxylase/reductase inhibitor 2-bromoethanesulfonic acid, sodium salt (BES), while growth on Eth was not CO2 dependent or BES sensitive. Although unable to promote growth, both propene and propene oxide (PrO)-induced expression of the genes encoding the alpha subunit of alkene monooxygenase (etnC) and epoxyethane CoM transferase (etnE) to similar levels as did Eth and EtO. Propene was transformed by Eth-grown and propene-grown/EtO-induced JS614 to PrO at a rate 4.2 times faster than PrO was consumed. As a result PrO accumulated in growth medium to 900 μM during EtO-induced growth on propene. PrO (50–100 μM) exerted inhibitory effects on growth of JS614 on both acetate and Eth, and on EtO-induced growth on Eth. However, higher EtO concentrations (300–400 μM) overcame the negative effects of PrO on Eth-dependent growth.

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Acknowledgments

We wish to thank Dr. Mohammad Azizian and Dr. Luis Sayavedra-Soto for valuable analytical support. Funding was provided by the NSF-IGERT program and by a research grant from the U.S. Environmental Protection Agency-sponsored Western Region Hazardous Substance Research Center under agreement R-828772. This article has not been reviewed by the agency, and no official endorsement should be inferred.

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Authors and Affiliations

  1. Oregon State University, Corvallis, OR, 97331, USA

    Anne E. Taylor, Daniel J. Arp, Peter J. Bottomley & Lewis Semprini

  2. Department of Crop and Soil Science, 3017 Agricultural Life Science Building, Oregon State University, Corvallis, OR, 97331, USA

    Anne E. Taylor

  3. School of Chemical, Biological and Environmental Engineering, 102 Gleeson Hall, Oregon State University, Corvallis, OR, 97331, USA

    Anne E. Taylor & Lewis Semprini

  4. Department of Botany and Plant Pathology, 2082 Cordley Hall, Oregon State University, Corvallis, OR, 97331, USA

    Daniel J. Arp

  5. Department of Microbiology, 220 Nash Hall, Oregon State University, Corvallis, OR, 97331, USA

    Peter J. Bottomley

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  1. Anne E. Taylor
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  4. Lewis Semprini
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Correspondence to Anne E. Taylor.

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Taylor, A.E., Arp, D.J., Bottomley, P.J. et al. Extending the alkene substrate range of vinyl chloride utilizing Nocardioides sp. strain JS614 with ethene oxide. Appl Microbiol Biotechnol 87, 2293–2302 (2010). https://doi.org/10.1007/s00253-010-2719-8

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  • DOI: https://doi.org/10.1007/s00253-010-2719-8

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